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-Structure paper
Title | Towards the application of Tc toxins as a universal protein translocation system. |
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Journal, issue, pages | Nat Commun, Vol. 10, Issue 1, Page 5263, Year 2019 |
Publish date | Nov 20, 2019 |
![]() | Daniel Roderer / Evelyn Schubert / Oleg Sitsel / Stefan Raunser / ![]() |
PubMed Abstract | Tc toxins are bacterial protein complexes that inject cytotoxic enzymes into target cells using a syringe-like mechanism. Tc toxins are composed of a membrane translocator and a cocoon that ...Tc toxins are bacterial protein complexes that inject cytotoxic enzymes into target cells using a syringe-like mechanism. Tc toxins are composed of a membrane translocator and a cocoon that encapsulates a toxic enzyme. The toxic enzyme varies between Tc toxins from different species and is not conserved. Here, we investigate whether the toxic enzyme can be replaced by other small proteins of different origin and properties, namely Cdc42, herpes simplex virus ICP47, Arabidopsis thaliana iLOV, Escherichia coli DHFR, Ras-binding domain of CRAF kinase, and TEV protease. Using a combination of electron microscopy, X-ray crystallography and in vitro translocation assays, we demonstrate that it is possible to turn Tc toxins into customizable molecular syringes for delivering proteins of interest across membranes. We also infer the guidelines that protein cargos must obey in terms of size, charge, and fold in order to apply Tc toxins as a universal protein translocation system. |
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Methods | EM (single particle) / X-ray diffraction |
Resolution | 2 - 5.1 Å |
Structure data | ![]() EMDB-10314: ![]() PDB-6sup: ![]() PDB-6suq: |
Chemicals | ![]() ChemComp-MG: ![]() ChemComp-HOH: |
Source |
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![]() | TOXIN / Toxins / Tc Toxins |