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TitleStructure-guided mutagenesis of the capsid protein indicates that a nanovirus requires assembled viral particles for systemic infection.
Journal, issue, pagesPLoS Pathog, Vol. 19, Issue 1, Page e1011086, Year 2023
Publish dateJan 9, 2023
AuthorsStefano Trapani / Eijaz Ahmed Bhat / Michel Yvon / Joséphine Lai-Kee-Him / François Hoh / Marie-Stéphanie Vernerey / Elodie Pirolles / Mélia Bonnamy / Guy Schoehn / Jean-Louis Zeddam / Stéphane Blanc / Patrick Bron /
PubMed AbstractNanoviruses are plant multipartite viruses with a genome composed of six to eight circular single-stranded DNA segments. The distinct genome segments are encapsidated individually in icosahedral ...Nanoviruses are plant multipartite viruses with a genome composed of six to eight circular single-stranded DNA segments. The distinct genome segments are encapsidated individually in icosahedral particles that measure ≈18 nm in diameter. Recent studies on the model species Faba bean necrotic stunt virus (FBNSV) revealed that complete sets of genomic segments rarely occur in infected plant cells and that the function encoded by a given viral segment can complement the others across neighbouring cells, presumably by translocation of the gene products through unknown molecular processes. This allows the viral genome to replicate, assemble into viral particles and infect anew, even with the distinct genome segments scattered in different cells. Here, we question the form under which the FBNSV genetic material propagates long distance within the vasculature of host plants and, in particular, whether viral particle assembly is required. Using structure-guided mutagenesis based on a 3.2 Å resolution cryogenic-electron-microscopy reconstruction of the FBNSV particles, we demonstrate that specific site-directed mutations preventing capsid formation systematically suppress FBNSV long-distance movement, and thus systemic infection of host plants, despite positive detection of the mutated coat protein when the corresponding segment is agroinfiltrated into plant leaves. These results strongly suggest that the viral genome does not propagate within the plant vascular system under the form of uncoated DNA molecules or DNA:coat-protein complexes, but rather moves long distance as assembled viral particles.
External linksPLoS Pathog / PubMed:36622854 / PubMed Central
MethodsEM (single particle)
Resolution3.19 Å
Structure data

10097

6s44

EMDB-10097, PDB-6s44:
Faba bean necrotic stunt virus (FBNSV)
Method: EM (single particle) / Resolution: 3.19 Å

Source
  • faba bean necrotic stunt virus
KeywordsVIRUS / icosahedral T1 capsid

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