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TitleMolecular basis for the assembly of RuBisCO assisted by the chaperone Raf1.
Journal, issue, pagesNat Plants, Vol. 6, Issue 6, Page 708-717, Year 2020
Publish dateMay 25, 2020
AuthorsLing-Yun Xia / Yong-Liang Jiang / Wen-Wen Kong / Hui Sun / Wei-Fang Li / Yuxing Chen / Cong-Zhao Zhou /
PubMed AbstractThe folding and assembly of RuBisCO, the most abundant enzyme in nature, needs a series of chaperones, including the RuBisCO accumulation factor Raf1, which is highly conserved in cyanobacteria and ...The folding and assembly of RuBisCO, the most abundant enzyme in nature, needs a series of chaperones, including the RuBisCO accumulation factor Raf1, which is highly conserved in cyanobacteria and plants. Here, we report the crystal structures of Raf1 from cyanobacteria Anabaena sp. PCC 7120 and its complex with RuBisCO large subunit RbcL. Structural analyses and biochemical assays reveal that each Raf1 dimer captures an RbcL dimer, with the C-terminal tail inserting into the catalytic pocket, and further mediates the assembly of RbcL dimers to form the octameric core of RuBisCO. Furthermore, the cryo-electron microscopy structures of the RbcL-Raf1-RbcS assembly intermediates enable us to see a dynamic assembly process from RbcLRaf1 to the holoenzyme RbcLRbcS. In vitro assays also indicate that Raf1 can attenuate and reverse CcmM-mediated cyanobacterial RuBisCO condensation. Combined with previous findings, we propose a putative model for the assembly of cyanobacterial RuBisCO coordinated by the chaperone Raf1.
External linksNat Plants / PubMed:32451445
MethodsEM (single particle) / X-ray diffraction
Resolution2.85 - 3.73 Å
Structure data

0959

6lrr

EMDB-0959, PDB-6lrr:
Cryo-EM structure of RuBisCO-Raf1 from Anabaena sp. PCC 7120
Method: EM (single particle) / Resolution: 3.37 Å

0960

6lrs

EMDB-0960, PDB-6lrs:
Cryo-EM structure of RbcL8-RbcS4 from Anabaena sp. PCC 7120
Method: EM (single particle) / Resolution: 3.37 Å

EMDB-0961:
The RuBisCO assembly intermediate RbcL-RbcS-Raf1 (L8S4F8)
Method: EM (single particle) / Resolution: 3.73 Å

EMDB-0962:
The cryo-EM structure of RuBisCO from Anabaena sp. PCC 7120
Method: EM (single particle) / Resolution: 3.67 Å

PDB-6kkm:
Crystal structure of RbcL-Raf1 complex from Anabaena sp. PCC 7120
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-6kkn:
Crystal structure of RuBisCO accumulation factor Raf1 from Anabaena sp. PCC 7120
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

Source
  • Nostoc sp. PCC 7120 (bacteria)
  • nostoc sp. (strain pcc 7120 / sag 25.82 / utex 2576) (bacteria)
KeywordsCHAPERONE/PHOTOSYNTHESIS / RuBisCO / Chaperone / Raf1 / RbcL / CHAPERONE-PHOTOSYNTHESIS complex / CHAPERONE/LYASE / CHAPERONE-LYASE complex / LYASE / PHOTOSYNTHESIS

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