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Structure paper

TitleTightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 958, Year 2019
Publish dateFeb 27, 2019
AuthorsYi Zhou / Sharmishtha Musalgaonkar / Arlen W Johnson / David W Taylor /
PubMed AbstractThe catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the ...The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.
External linksNat Commun / PubMed:30814529 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 3.8 Å
Structure data

0369

6n8j

EMDB-0369, PDB-6n8j:
Cryo-EM structure of late nuclear (LN) pre-60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.5 Å

0370

6n8k

EMDB-0370, PDB-6n8k:
Cryo-EM structure of early cytoplasmic-immediate (ECI) pre-60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.6 Å

0371

6n8l

EMDB-0371, PDB-6n8l:
Cryo-EM structure of early cytoplasmic-late (ECL) pre-60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.6 Å

0372

6n8m

EMDB-0372, PDB-6n8m:
Cryo-EM structure of pre-Lsg1 (PL) pre-60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.5 Å

0373

6n8n

EMDB-0373, PDB-6n8n:
Cryo-EM structure of Lsg1-engaged (LE) pre-60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.8 Å

0374

6n8o

EMDB-0374, PDB-6n8o:
Cryo-EM structure of Rpl10-inserted (RI) pre-60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / ribosome biogenesis / Nmd3 / peptidyl transferase center

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