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TitleNew tools for automated high-resolution cryo-EM structure determination in RELION-3.
Journal, issue, pagesElife, Vol. 7, Year 2018
Publish dateNov 9, 2018
AuthorsJasenko Zivanov / Takanori Nakane / Björn O Forsberg / Dari Kimanius / Wim Jh Hagen / Erik Lindahl / Sjors Hw Scheres /
PubMed AbstractHere, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory ...Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, model generation and 3D-classification. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. We illustrate these developments with publicly available data sets: together with a Bayesian approach to beam-induced motion correction it leads to resolution improvements of 0.2-0.7 Å compared to previous RELION versions.
External linksElife / PubMed:30412051 / PubMed Central
MethodsEM (single particle)
Resolution1.65 - 3.1 Å
Structure data

EMDB-0144:
Human apo-ferritin reconstructed in RELION-3.0
Method: EM (single particle) / Resolution: 1.65 Å

EMDB-0152:
RELION-3.0 reconstruction of influenza hemagglutinin (HA) trimer using particles from micrographs tilted at 40 degrees in EMPIAR-10097
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-0153:
RELION-3.0 reconstruction of beta-galactosidase particles in EMPIAR-10061
Method: EM (single particle) / Resolution: 1.9 Å

EMDB-0263:
Horse spleen apo-ferritin with beam tilt correction in RELION-3.0
Method: EM (single particle) / Resolution: 2.18 Å

Source
  • Escherichia coli (E. coli)
  • Homo sapiens (human)
  • Escherichia coli K-12 (bacteria)
  • Equus caballus (horse)

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