EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural atlas of the intact jumbo phage phiKZ.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年4月13日
著者	Hao Xiao / Zeng Peng / Junquan Zhou / Yuan Chen / Yuning Peng / Yixiong Tang / Tao Li / Wenyuan Chen / Sheng-You Huang / Lingpeng Cheng / Hongrong Liu /
PubMed 要旨	Jumbo phage phiKZ, a key model for studying phage nucleus formation and bacterial defense mechanisms, possesses a highly complex tail machine that is essential for infection. Here, we present the ...Jumbo phage phiKZ, a key model for studying phage nucleus formation and bacterial defense mechanisms, possesses a highly complex tail machine that is essential for infection. Here, we present the structural atlas of the intact jumbo phage phiKZ by cryo-EM, thereby identifying 40 constituent proteins and unveiling its modular architecture. The virion, with a length of approximately 360 nm, is comprised of an icosahedral capsid of 2520 polypeptide chains from 11 proteins, and a massive tail machine of over 900 polypeptide chains from 29 proteins. The tail features a unique, multi-layered neck and a highly elaborate baseplate. The neck is reinforced by whisker-like proteins and anchors the contractile tail, which terminates in the baseplate. The baseplate is constituted by a central hub, an inner periphery of interlocking wedge heterotrimers and hexagonal rings, and an outer periphery with a striking hexagonal star configuration. This intricate peripheral region of the baseplate serves as an extended platform for twelve peripheral fibers, which mediate host cell adsorption. Our findings provide a structural framework for understanding jumbo phage assembly and infection, thus contributing to the foundation for future functional studies and rational engineering of these phages for potential therapeutic applications.
リンク	Nat Commun / PubMed:41974715
手法	EM (単粒子)
解像度	3.15 - 7.61 Å
構造データ	67095 9xpe EMDB-67095, PDB-9xpe: Structure of the Portal and Adaptor Proteins of the Phage Phikz 手法: EM (単粒子) / 解像度: 3.37 Å 67096 9xpf EMDB-67096, PDB-9xpf: The structure of sheath and tube proteins of phage Phikz 手法: EM (単粒子) / 解像度: 3.15 Å 67097 9xpg EMDB-67097, PDB-9xpg: The structure of gp139 protein of phage phikz 手法: EM (単粒子) / 解像度: 4.14 Å 67098 9xph EMDB-67098, PDB-9xph: The structure of baseplate central region of phage phikz 手法: EM (単粒子) / 解像度: 4.02 Å 67102 9xps EMDB-67102, PDB-9xps: The neck structure of the Phage Phikz 手法: EM (単粒子) / 解像度: 3.52 Å 67111 9xqd EMDB-67111, PDB-9xqd: The structure of outer peripheral region in the phage phiKZ baseplate complex 手法: EM (単粒子) / 解像度: 4.53 Å 67121 9xqs EMDB-67121, PDB-9xqs: Structure of the inner peripheral region in the phage phiKZ baseplate complex 手法: EM (単粒子) / 解像度: 3.65 Å EMDB-67123: Map of the unit1 in the phage phiKZ baseplate complex 手法: EM (単粒子) / 解像度: 4.39 Å EMDB-67124: Map of the unit2 in the phage phiKZ baseplate complex 手法: EM (単粒子) / 解像度: 4.55 Å EMDB-67125: The overall tail of phage PhiKZ 手法: EM (単粒子) / 解像度: 7.61 Å
由来	pseudomonas phage phikz (ファージ)
キーワード	VIRAL PROTEIN / portal / adaptor / sheath / tube / baseplate / inner peripheral / spike / neck / outer peripheral