EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A family of ribosome hibernation factors widespread in Archaea.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年4月27日
著者	Clément Madru / Gabrielle Bourgeois / Rémi Dulermo / Régine Capeyrou / Gwendoline Joncour / Karima Figuigui / Magalie Duchateau / Julia Chamot-Rooke / Claire Duboc / Stéphane l'Haridon / Logan Mc Teer / Marta Kwapisz / Béatrice Clouet-d'Orval / Marie Bouvier / Yves Mechulam / Guillaume Borrel / Emmanuelle Schmitt / Didier Flament /
PubMed 要旨	Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Using cryo-EM analysis, we studied hibernation pathways in Pyrococcus abyssi ...Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Using cryo-EM analysis, we studied hibernation pathways in Pyrococcus abyssi stressed cells. We identified HibA, a previously unrecognized family of hibernation factors widespread in Archaea. HibA consists of a bacterial-like HPF/RaiA domain fused to a Cystathionine Beta Synthase module. Unexpectedly, HibA binds to the ribosome in three different conformations, occupying the A, P and E sites of tRNAs, as well as that of mRNA, enhancing its ability to protect the ribosome from degradation. Idle ribosomes also frequently accumulate the archaeal homolog of eukaryotic ribosome maturation protein SBDS (aSBDS), suggesting that stressed archaeal cells may engage parallel hibernation routes in which aSBDS can complement HibA. Deletion of hibA in Thermococcus barophilus delays recovery from stationary phase and reduces 70S ribosome pools, establishing its role in ribosome preservation. Taxonomic profiling shows that many archaeal lineages encode distinct repertoires of ribosome-associated protection factors, underscoring the modular and multi-layered nature of archaeal hibernation systems. In addition, a comprehensive phylogenetic analysis highlights the evolutionary relationships between prevalent ribosome hibernation factors across Bacteria and Archaea.
リンク	Nat Commun / PubMed:42045235 / PubMed Central
手法	EM (単粒子)
解像度	2.1 - 2.3 Å
構造データ	55135 9sra EMDB-55135, PDB-9sra: Cryo-EM structure of P. abyssi HibA:ribosome with an SD:antiSD duplex 手法: EM (単粒子) / 解像度: 2.2 Å 55136 9srb EMDB-55136, PDB-9srb: Cryo-EM structure of P. abyssi 70S ribosome in complex with hibernation factor HibA and SBDS 手法: EM (単粒子) / 解像度: 2.3 Å 55137 9src EMDB-55137, PDB-9src: Cryo-EM structure of P. abyssi 70S ribosome in complex with hibernation factor HibA in PTC conformation 手法: EM (単粒子) / 解像度: 2.1 Å 55138 9srd EMDB-55138, PDB-9srd: Cryo-EM structure of P. abyssi 70S ribosome in complex with hibernation factor HibA (HibA-uL5 conformation) 手法: EM (単粒子) / 解像度: 2.1 Å 55139 9sre EMDB-55139, PDB-9sre: Cryo-EM structure of P. abyssi 70S ribosome in complex with hibernation factor HibA (PTC conformation with E-site tRNA) 手法: EM (単粒子) / 解像度: 2.11 Å 55636 9t7h EMDB-55636, PDB-9t7h: Cryo-EM structure of P. abyssi 70S ribosome in complex with hibernation factor HibA (L1 stalk conformation) 手法: EM (単粒子) / 解像度: 2.1 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-HOH: WATER
由来	pyrococcus abyssi ge5 (古細菌)
キーワード	RIBOSOME / 70S / Hibernation / Pyrococcus abyssi