EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Global conformations of complex I are distinguished by the binding of a unique interdomain bridging subunit.
ジャーナル・号・ページ	Sci Adv, Vol. 11, Issue 40, Page eadz0693, Year 2025
掲載日	2025年10月3日
著者	Chris Seunggyu Lee / Daniel N Grba / John J Wright / Bozhidar S Ivanov / Judy Hirst /
PubMed 要旨	Complex I (CI; NADH ubiquinone oxidoreductase) is central to energy generation and metabolic homeostasis in mammalian cells but contributes to adverse outcome pathways under challenging conditions. ...Complex I (CI; NADH ubiquinone oxidoreductase) is central to energy generation and metabolic homeostasis in mammalian cells but contributes to adverse outcome pathways under challenging conditions. During ischemia, mammalian CI transitions from a turnover-ready, structurally "closed" state toward a dormant "open" state that prevents it from functioning in reverse during reperfusion to produce reactive oxygen species. Unfortunately, simpler, genetically tractable CI models do not recapitulate the same regulatory behavior, compromising mechanistic studies. Here, we report the structure of isolated CI from the yeast (-CI) and identify distinct closed and open states that resemble those of mammalian CI. Notably, a hitherto-unknown protein (NUQM) completes an interdomain bridge in only the closed state, implying that NUQM stabilizes it by restricting the conformational changes of opening. The direct correlation of NUQM binding with closed/open status in -CI provides opportunities for investigating regulatory mechanisms relevant to reversible catalysis and ischemia-reperfusion injury.
リンク	Sci Adv / PubMed:41032597 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 20.0 Å
構造データ	52875 9iho EMDB-52875, PDB-9iho: Open state without NUQM and without flavoprotein (classification state 4) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs 手法: EM (単粒子) / 解像度: 3.8 Å 52876 9ihp EMDB-52876, PDB-9ihp: Open state without NUQM and with flavoprotein (classification state 3) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs 手法: EM (単粒子) / 解像度: 3.34 Å 52877 9ihq EMDB-52877, PDB-9ihq: Closed state with NUQM and without flavoprotein (classification state 2) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs 手法: EM (単粒子) / 解像度: 2.9 Å 52878 9ihr EMDB-52878, PDB-9ihr: Closed state with NUQM and with flavoprotein (classification state 1) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-52890: NUQM-free state of Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes 手法: EM (単粒子) / 解像度: 8.2 Å EMDB-52891: NUQM-bound state of Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-52892: Flavoprotein (NUHM and NUBM)-free state of Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-52893: Flavoprotein (NUHM and NUBM)-containing state of Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes 手法: EM (単粒子) / 解像度: 4.7 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-PLC: DIUNDECYL PHOSPHATIDYL CHOLINE / リン脂質YM ChemComp-K: Unknown entry ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / リン脂質YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-ZN: Unknown entry ChemComp-EHZ: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN*: FLAVIN MONONUCLEOTIDE
由来	komagataella pastoris (菌類) Komagataella phaffii (菌類)
キーワード	MEMBRANE PROTEIN / NADH:ubiquinone oxidoreductase nanodisc membrane protein complex