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- EMDB-52876: Open state without NUQM and with flavoprotein (classification sta... -

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Basic information

Entry
Database: EMDB / ID: EMD-52876
TitleOpen state without NUQM and with flavoprotein (classification state 3) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs
Map dataFinal consensus map used for model building and model refinement. Globally sharpened with RELION postprocess.
Sample
  • Complex: Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NADH:ubiquinone oxidoreductase)
    • Protein or peptide: x 43 types
  • Ligand: x 10 types
KeywordsNADH:ubiquinone oxidoreductase nanodisc membrane protein complex / MEMBRANE PROTEIN
Function / homology
Function and homology information


NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase complex / oxidoreductase activity, acting on NAD(P)H / NADH dehydrogenase activity / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly ...NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase complex / oxidoreductase activity, acting on NAD(P)H / NADH dehydrogenase activity / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / aerobic respiration / respiratory electron transport chain / electron transport chain / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / oxidoreductase activity / mitochondrial inner membrane / protein-containing complex binding / mitochondrion / metal ion binding / membrane
Similarity search - Function
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / : ...NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / : / SLBB domain / : / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-quinone oxidoreductase, chain M/4 / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / NADH ubiquinone oxidoreductase, F subunit / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH dehydrogenase, subunit C / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / Complex 1 LYR protein domain / NADH:ubiquinone oxidoreductase / Complex 1 protein (LYR family) / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / : / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / NADH:quinone oxidoreductase/Mrp antiporter, TM / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / 2Fe-2S iron-sulfur cluster binding domain / NAD-dependent epimerase/dehydratase / NuoE domain / NAD dependent epimerase/dehydratase family / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / NADH-quinone oxidoreductase subunit E-like / NADH:ubiquinone oxidoreductase, 51kDa subunit, conserved site / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. / NADH-quinone oxidoreductase subunit E, N-terminal
Similarity search - Domain/homology
Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / Uncharacterized protein / NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) ...Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / Uncharacterized protein / NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUJM (B14.7) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / BA75_00622T0 / NULM (ND4L) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUMM (13 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH-ubiquinone oxidoreductase / NUXM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / BA75_00589T0 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NIAM (ASHI) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NIDM (PDSW) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / BA75_05084T0 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NI8M (B8) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NI9M (B9) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NB2M (B12) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / BA75_04796T0 / NB5M (B15) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NESM (ESSS) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / Acyl carrier protein / Acyl carrier protein / NUSM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUTM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUUM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
Similarity search - Component
Biological speciesKomagataella pastoris (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.34 Å
AuthorsGrba DN / Hirst J
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UU_00015/2 United Kingdom
CitationJournal: Sci Adv / Year: 2025
Title: Global conformations of complex I are distinguished by the binding of a unique interdomain bridging subunit.
Authors: Chris Seunggyu Lee / Daniel N Grba / John J Wright / Bozhidar S Ivanov / Judy Hirst /
Abstract: Complex I (CI; NADH ubiquinone oxidoreductase) is central to energy generation and metabolic homeostasis in mammalian cells but contributes to adverse outcome pathways under challenging conditions. ...Complex I (CI; NADH ubiquinone oxidoreductase) is central to energy generation and metabolic homeostasis in mammalian cells but contributes to adverse outcome pathways under challenging conditions. During ischemia, mammalian CI transitions from a turnover-ready, structurally "closed" state toward a dormant "open" state that prevents it from functioning in reverse during reperfusion to produce reactive oxygen species. Unfortunately, simpler, genetically tractable CI models do not recapitulate the same regulatory behavior, compromising mechanistic studies. Here, we report the structure of isolated CI from the yeast (-CI) and identify distinct closed and open states that resemble those of mammalian CI. Notably, a hitherto-unknown protein (NUQM) completes an interdomain bridge in only the closed state, implying that NUQM stabilizes it by restricting the conformational changes of opening. The direct correlation of NUQM binding with closed/open status in -CI provides opportunities for investigating regulatory mechanisms relevant to reversible catalysis and ischemia-reperfusion injury.
History
DepositionFeb 21, 2025-
Header (metadata) releaseOct 15, 2025-
Map releaseOct 15, 2025-
UpdateOct 15, 2025-
Current statusOct 15, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_52876.png

Downloads & links

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Map

FileDownload / File: emd_52876.map.gz / Format: CCP4 / Size: 600.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFinal consensus map used for model building and model refinement. Globally sharpened with RELION postprocess.
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 540 pix.
= 501.66 Å		0.93 Å/pix.
x 540 pix.
= 501.66 Å		0.93 Å/pix.
x 540 pix.
= 501.66 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.929 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.026769355 - 0.07586619
Average (Standard dev.)-0.00010022779 (±0.0022485908)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions540540540
Spacing540540540
CellA=B=C: 501.66 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_52876_msk_1.map
Projections & Slices
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Half map: Half map 2

Fileemd_52876_half_map_1.map
AnnotationHalf map 2
Projections & Slices
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Half map: Half map 1

Fileemd_52876_half_map_2.map
AnnotationHalf map 1
Projections & Slices
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Sample components

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Entire : Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NA...

EntireName: Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NADH:ubiquinone oxidoreductase)
Components
  • Complex: Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NADH:ubiquinone oxidoreductase)
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: BA75_00622T0
    • Protein or peptide: NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
    • Protein or peptide: NUAM (75 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: NULM (ND4L) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
    • Protein or peptide: NUXM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
    • Protein or peptide: NUMM (13 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NI8M (B8) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: BA75_04796T0
    • Protein or peptide: NADH-ubiquinone oxidoreductase
    • Protein or peptide: NUJM (B14.7) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
    • Protein or peptide: NI9M (B9) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: BA75_00589T0
    • Protein or peptide: Pichia pastoris NADH-ubiquinone oxidoreductase subunit NEBM
    • Protein or peptide: BA75_05084T0
    • Protein or peptide: NUTM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NESM (ESSS) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NUSM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NUUM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NB2M (B12) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NIAM (ASHI) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NB5M (B15) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
    • Protein or peptide: NIDM (PDSW) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: DIUNDECYL PHOSPHATIDYL CHOLINE
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: POTASSIUM ION
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: CARDIOLIPIN
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: ZINC ION
  • Ligand: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

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Supramolecule #1: Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NA...

SupramoleculeName: Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NADH:ubiquinone oxidoreductase)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#43
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 909 KDa

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Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 16.019821 KDa
SequenceString:
(FME)LQDLFNNYV ILVGILGLIF LGVNYFIVES PRMDENNGNI SDYIEKSGPF ECGFSSFEQS HNPIPIAFIL VALLFL PFD LEVSSMLPYI VSIYSVGIYG LIIFILFLLI LIVGFIYEFN TKSLSITTIL HKKNKALVKN LY

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

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Macromolecule #2: BA75_00622T0

MacromoleculeName: BA75_00622T0 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 23.353986 KDa
SequenceString: MFVPCRTQIS RSLAHSVRYA SKQVATSHSY SSDLPTDIPV LSKRPHTNLL DYTFTTFDKM KNWARKSSFW PMTFGLACCA VEMMHVSAP RYDQDRLGII FRASPRQSDI MIVAGTVTNK MAPALRQVYD QMPYPRWVIS MGSCANGGGY YHYSYSVVRG V DRIVPVDI ...String:
MFVPCRTQIS RSLAHSVRYA SKQVATSHSY SSDLPTDIPV LSKRPHTNLL DYTFTTFDKM KNWARKSSFW PMTFGLACCA VEMMHVSAP RYDQDRLGII FRASPRQSDI MIVAGTVTNK MAPALRQVYD QMPYPRWVIS MGSCANGGGY YHYSYSVVRG V DRIVPVDI YVPGCPPTSE ALMYGVFQLQ KKMMDGQTHR MWYRSY

UniProtKB: BA75_00622T0

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Macromolecule #3: NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreduc...

MacromoleculeName: NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 33.18368 KDa
SequenceString: MIAARSIQRA IGTKPLAVRA VVLTTNSFRG FSKSSMSRKA DEEPLPVLRD LPRPEYGELH APVYNPAEKY KEQIEELHKF GRYIMGCLP KFVQQFSVWK DELVIYVAPS ALTQVATFLK DHTSAQFKAC MDVTAADYPT RTNRFDVVYN LLSVRHNSRI R IKTYASEV ...String:
MIAARSIQRA IGTKPLAVRA VVLTTNSFRG FSKSSMSRKA DEEPLPVLRD LPRPEYGELH APVYNPAEKY KEQIEELHKF GRYIMGCLP KFVQQFSVWK DELVIYVAPS ALTQVATFLK DHTSAQFKAC MDVTAADYPT RTNRFDVVYN LLSVRHNSRI R IKTYASEV SPVPSVVPLF QGANWFERET YDLFGIFFEG HPDLRRIMTD YGFQGHPLRK DFPTTGYTEV RYDAEKRRVV YE PLELTQA WRNFTVGSSV WEQVGDGKDF TPESFKLPTP APDPEPEADK KK

UniProtKB: NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #4: NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreduc...

MacromoleculeName: NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 54.962457 KDa
SequenceString: MFARLHAKRG WKSLARARVG SRFLSNSSIA RAAQTKDNND FYDVKTKKND FFPVQVDGIE SSLGKYEEFA KDAHEWESWN LQNEDHPEY PVKRTKIRHF TLNFGPQHPA AHGVLRLILE LHGEEILRSD PHVGLLH(2MR)GT EKLIESKTYM QALPYFD RL DYVSMMTNEQ ...String:
MFARLHAKRG WKSLARARVG SRFLSNSSIA RAAQTKDNND FYDVKTKKND FFPVQVDGIE SSLGKYEEFA KDAHEWESWN LQNEDHPEY PVKRTKIRHF TLNFGPQHPA AHGVLRLILE LHGEEILRSD PHVGLLH(2MR)GT EKLIESKTYM QALPYFD RL DYVSMMTNEQ VFSLAVEKLL NLEVPERGQY IRTLFGEITR VLNHLMSVLS HAMDVGALTP FLWGFEEREK LMEFYERV S GARLHSAYVR PGGVSQDLPA GLLDDIYMWA TQFGDRLDET EELLTDSRIW KQRTIGIGEV TAEDALNYGL SGVMLRGSG VPFDIRKSQP YDAYDKVDFD IAVGTKGDCY DRYLIRMTEF RQSLRIIEQC CNQMPPGPVK VEDFKVAPPP RALMKEDMEA LIHHFLLYT KGYAVPAGET YTAIEAPKGE MAVYVVSDGS ERPYRCKIRA PGFAHLGAFD HIARGHFLPD AVAIIGTMDL V FGEVDR

UniProtKB: NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #5: NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreduc...

MacromoleculeName: NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 27.396691 KDa
SequenceString: MSLLRLLPKA TKPLQQRIVT VTPKRFSHMI SVHRDTPKDN PEIPFEFTKE NLERAKEIIA KYPPQYKKGA VMPLLDLGQR QLGFTSISV MNYVAKYLDM PPMRVYEVAT FYTMYNRKPM GKYNVQVCTT TPCQLCGSDG IMKAITEHLQ IRPGQTTPDN L FTLQEVEC ...String:
MSLLRLLPKA TKPLQQRIVT VTPKRFSHMI SVHRDTPKDN PEIPFEFTKE NLERAKEIIA KYPPQYKKGA VMPLLDLGQR QLGFTSISV MNYVAKYLDM PPMRVYEVAT FYTMYNRKPM GKYNVQVCTT TPCQLCGSDG IMKAITEHLQ IRPGQTTPDN L FTLQEVEC LGACVNAPMI AVNDDFYEDL TPERTVEILK GFQSGDIPKP GPADRHSCEP HSGPKVLTNE TPYHVKDFIR DD L

UniProtKB: NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 52.23466 KDa
SequenceString: MLRQQVGRKL VSKRFFSGLK DSDRIFQNVY SKYGEDLKSS QQRGDWYKTK EIILKGHEWL INELKASGLR GRGGAGFPSG LKYSFMPPN PNREPQYLVV NADEGEPGTC KDREIIRKEP HKLVEGCLLA GRAMNATAAY IYIRGEFYNE AVALQTAINE A YKAGLLGK ...String:
MLRQQVGRKL VSKRFFSGLK DSDRIFQNVY SKYGEDLKSS QQRGDWYKTK EIILKGHEWL INELKASGLR GRGGAGFPSG LKYSFMPPN PNREPQYLVV NADEGEPGTC KDREIIRKEP HKLVEGCLLA GRAMNATAAY IYIRGEFYNE AVALQTAINE A YKAGLLGK NACGSGYDFD VYIHRGMGAY ICGEETALIE SIEGKAGKPR LKPPFPAGVG LFGRPTTVTN VETVAVTPTI FR RSGAWFA SFGRDRNQGT KLFCISGHVN EPCTVEESMS IPLKELLEKH CGGIKGGWDN LLGVVPGGSS VPIMPKHVCE DVL MDFDAL KDVGSGLGTA AVIVLNKQTD IIRAIQRFSA FYKHESCGQC TPCREGTTYL QRMMDRFVTG QAKEKEIDLI YELS KEIEG HTICALGDAA AWPVQGLIKS FRPEMVERIK QYEAKLGESV SYGGWTSKGK VLDGKVVSNP IPHDAHHH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

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Macromolecule #7: NUAM (75 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreduc...

MacromoleculeName: NUAM (75 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 79.646414 KDa
SequenceString: MLSVKRSISR IPTARLFSTT RRQLKEVELT VDGVKVSIEA GSSIIQAAEK AGVTIPRYCY HEKLAVAGNC RMCLVEIERS PKLAASCAM PVQNGMSVLT NTPKIKKARE GVTEMLLENH PLDCPVCDQG GECDLQEQSL RYGSDRGRFH EIVGKRAVEN K AIGPLIKT ...String:
MLSVKRSISR IPTARLFSTT RRQLKEVELT VDGVKVSIEA GSSIIQAAEK AGVTIPRYCY HEKLAVAGNC RMCLVEIERS PKLAASCAM PVQNGMSVLT NTPKIKKARE GVTEMLLENH PLDCPVCDQG GECDLQEQSL RYGSDRGRFH EIVGKRAVEN K AIGPLIKT SMNRCIHCTR CVRFLNDVAG APEFGTAGRG NDMQIGTYVE RNVNSELSAN VIDLCPVGAL TSKPYAFRAR PW ELKRTET IDVHDALGSN VRVDTRGIEV MRVLPRLNDE INEEWISDKT RFACDGLKTQ RLTKPLIRQG NTFVDATWEE ALA TIAEGF QKVQPKENEI KAIVGSLTDL ESIVALKDLV NKLGSENVST DGDATTVPPA HGADFRSNYI FNSTIDNIEE ADQI LLVGT NPRHEAAVLN ARIRKVWLRS DLEVHHVGEE FDSTFELNHL GSDVNALAKA LTGEVGKKLS EASKPLIIIG SGVTE TQDA EAVYATVAKF VTSNANIVTP EWNGFNLLHR EASRVGALDV GFTTQSPEVA NTKAKFIYLL GADEIVNKDI PKDAFV VYQ GHHGDLGASF ADVILPGSAY TEKTATYVNT EGRVQSTRAA TNPPGVARED WMIIRALSEY LGATLPYDNL YEMRSRL AD VAPHLLRHEV VEPVSEGIAK VGFQSLVKPG IQSKGVLLKN PIENFYFTDV ISKSSPTMAK CVGSFGSKIE KIKDEKAN S YLSFA

UniProtKB: NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial

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Macromolecule #8: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 39.698844 KDa
SequenceString: (FME)LNNLFDFIE YIEYWLIIVA FVASVLIVVA YLTVAERKTM GYMQRRLGPN AVGYYGVLMA VADALKLLSK EIVLPH NGD IIYVMSGPLI SLFSVLLSWA VIPFGPGLSL LDSEYSIIYL LASGSIGVFG TVIVGWMSNS KYTVLATVRT TAQLISY EL VLTTVVFIIA ...String:
(FME)LNNLFDFIE YIEYWLIIVA FVASVLIVVA YLTVAERKTM GYMQRRLGPN AVGYYGVLMA VADALKLLSK EIVLPH NGD IIYVMSGPLI SLFSVLLSWA VIPFGPGLSL LDSEYSIIYL LASGSIGVFG TVIVGWMSNS KYTVLATVRT TAQLISY EL VLTTVVFIIA LIVSSLNINV IIESQYNIWY IIPFFPLCLI FFISALAETA RPPFDNVEAE SELVSGHMTE LSASPFVI F FLSEYCSMVL MSTLTAIFFF GGYLPFSNTI HHLILNLFDQ HSIYYFIIEG ILLSGYLAIK ANFFMFSFVW IRAAAPRLR YDLLILFCWY VLLPIVFAIV VFAPGILYCF DALPVII

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

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Macromolecule #9: NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreducta...

MacromoleculeName: NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 25.544215 KDa
SequenceString: MFRPLALKPS CRAINRIPVC RFSTGLIAQK PTLALTSGSI QGTHPEGFRL PRPKTWEESS ESALSKATKW YLLSEIFRGL YITLEMYFR APYTIYYPFE KGPVSPRFRG EHALRRYPSG EERCIACKLC EAVCPAQAIT IEAEERIDGS RRTYKYDIDM T KCIYCGYC ...String:
MFRPLALKPS CRAINRIPVC RFSTGLIAQK PTLALTSGSI QGTHPEGFRL PRPKTWEESS ESALSKATKW YLLSEIFRGL YITLEMYFR APYTIYYPFE KGPVSPRFRG EHALRRYPSG EERCIACKLC EAVCPAQAIT IEAEERIDGS RRTYKYDIDM T KCIYCGYC QESCPVDAIV ETPNVEYATE TREELLYNKE KLLANGDKWE QEIQYALDAD APYR

UniProtKB: NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #10: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 18.564352 KDa
SequenceString:
(FME)NLEILDLSI LGFYSLISSN SFESIAILVA TIMPVITELW YNYSQLFALL YTLIYVGAVV ILFVFILSVI NEKEEY EYE YSGLFILILV LLLDDLDDNE SNDNDYNNVY ITDYADWFNN YNESDLSVIG NLLFTEYALL LLIISFILLM SIIGLIL IV KKD

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

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Macromolecule #11: NULM (ND4L) subunit of mitochondrial NADH:ubiquinone oxidoreducta...

MacromoleculeName: NULM (ND4L) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 9.118741 KDa
SequenceString:
(FME)DLILLMLVA VGLNIIDIYI MIEILIMGCT VNTIYSASLD NDMIGLIYSL IQIIIAGVES AIGLSILVNY NRIRNS EEI ENE

UniProtKB: NULM (ND4L) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #12: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 72.43607 KDa
SequenceString: (FME)ILLCIFTPF LGSLISGFFG RYIGVNSSKW IVCLSIFVSV VSTYILFIDV IFNEIEYTYN IMNWLNVEYL VVDWSF TID KLSVSMLVPV VTVSFLVHLY SVSYMSHDPH QQRFFSYLSM FTFAMIVLVT GDNYLILFVG WEFIGVASYL LISFWFT RF NAVKSGLSAL ...String:
(FME)ILLCIFTPF LGSLISGFFG RYIGVNSSKW IVCLSIFVSV VSTYILFIDV IFNEIEYTYN IMNWLNVEYL VVDWSF TID KLSVSMLVPV VTVSFLVHLY SVSYMSHDPH QQRFFSYLSM FTFAMIVLVT GDNYLILFVG WEFIGVASYL LISFWFT RF NAVKSGLSAL LINRFGDALL VLGLSYLVLV TGSVNYNTVF ALGSYLDVNM ITVILICFLV ASMAKSAQLG LHSWLLLA M EGPTPVSSLL HAACLVIAGV FLLMRSSPLL EYTPIVLLLI LWLGGLSTLI SGLIAMVSND IKKIIALSTM SQVGIMMIA IGVSCYNLAL FHLICHSFFK ALLFMSAGSV IHSIISEYQD IRMYGGFRQF IPLAYTCIFI ASLSLMAIPG LTGFYSKDII IESMYGEYT FSGFIIYWFA LISATCTSLY SIRLIYYTFL NNPNSPKYIY INLKENDIVL ITPMIILTIL SIFGGYLLRD I YLGMGSPF NELFIHPNNL SLIETEFSLP SILKVLPLIT GLGGGLILLL IYEFNYSLFL VYNNKNLSKL YSFFNQKIMF DQ LLNNLIL RSGLNIGWLL NVHIDRGILQ LFGPLGLWKL STFISSKLNK LVFLNIGFIL EIIVLSWLLI VFKVSLLKYI LLL IIFIIL

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

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Macromolecule #13: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 54.784211 KDa
SequenceString: (FME)LVQNLIIFN IIGIVLLVLL NNNNNGILNK KSLLTIKNEL KLKQIGLIIS IINILIIIEN WIGFNSNYYG FQNIER FLN IYWGIDGISL LLVLLTLILG PIAILSNWKN LKGYYTYYVI SINMLVILMA LNFICLDLVS FYILFESTLA PLFLLIG IY GANNKNKAAY ...String:
(FME)LVQNLIIFN IIGIVLLVLL NNNNNGILNK KSLLTIKNEL KLKQIGLIIS IINILIIIEN WIGFNSNYYG FQNIER FLN IYWGIDGISL LLVLLTLILG PIAILSNWKN LKGYYTYYVI SINMLVILMA LNFICLDLVS FYILFESTLA PLFLLIG IY GANNKNKAAY YVLIYTLASS LFMLLSIVIY YIIINSTDYI YINNIILSIN LQSLLFIGII IGIAVKTPLM PVHTWLPT V HSESPLGGSI LLAGVILKLA IYGVIRLLLP NLSEISMMFT PIIYIISILT LIYSSLITLK QTDLKVIIAY SSISHLAVC MIGIFANNLI GINGSLILCL AHGFVSPALF ILVGGILYDR YHNRLINYYQ GLSTFMPIFS IYLVVFSFCN TGTPLSGNFV GEFLSITGA VIRHPIIGSL TAASVLLSAC YQMKLTNRLT GSVFSQYLHI TNDVTNREIF LLNWLLIPTI IIGIYPSFVT N ILNLASST LIYSVLI

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

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Macromolecule #14: NADH-ubiquinone oxidoreductase chain 2

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 59.097926 KDa
SequenceString: (FME)LFTSLLLII VYGSLVNKSH LLTKLINRLG LIIIIYTLLI HLNINTLENL SIGINMFNNL LLINSFNNTI IELILI ICI LYVLLISFNI TGLSIIDNLN YSKRTFIVIV LFNIIGLTLL PLVNDIMILF IIIELQSYSL YLLTAIFNES HNSTKSA LY YFIVGSIASF ...String:
(FME)LFTSLLLII VYGSLVNKSH LLTKLINRLG LIIIIYTLLI HLNINTLENL SIGINMFNNL LLINSFNNTI IELILI ICI LYVLLISFNI TGLSIIDNLN YSKRTFIVIV LFNIIGLTLL PLVNDIMILF IIIELQSYSL YLLTAIFNES HNSTKSA LY YFIVGSIASF IILDATVSIY DDLGLTNIQY ILMYIDKLEI FDLLCLLFGL FIKIGLAPFY SYSITIYSLS PTIITNYI S LMPKLSILTL IYIIMHYINI VNNTDLNYVI SFLILISMFI GSFGGLNVIR IKTLLAYSSL LNVSYMLLAI LSNSNESII AYIYYIIQYS LTHINIFSII LVISIYLPIM NNNITSNTVD NKYSNNGGNM LNVMITKYSP IEYINQLKNL FINNIFIAIA LCISIFSLI GIPPLTGFYG KYLVLISGLN NGYVLLSIVL IIASSISTVY YATILKVLSF DTLSFKDIIS NNLIINTSPE N NNNEIKPE ISNVISYVIS SLTLIILLII IQYENIVKGA YLVLLYNWV

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

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Macromolecule #15: NUXM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Com...

MacromoleculeName: NUXM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 22.263098 KDa
SequenceString: (ACE)METYELIDS DPKFSRVVRY FRPSDYLTWA LGTIGAPGLM VLFEKIEPAK GNTFKMPPPS IMRIATTIGF FGGFYY AYT SSTKRFWGYS ENAKEVAKDR YEVKKALSEK KSPYGSSLLN PYQQDMSARN STNSQLLLAI FPWFNVANHQ SHGIDLR KY YEVRDGEENW ...String:
(ACE)METYELIDS DPKFSRVVRY FRPSDYLTWA LGTIGAPGLM VLFEKIEPAK GNTFKMPPPS IMRIATTIGF FGGFYY AYT SSTKRFWGYS ENAKEVAKDR YEVKKALSEK KSPYGSSLLN PYQQDMSARN STNSQLLLAI FPWFNVANHQ SHGIDLR KY YEVRDGEENW NFTLPPLDQV KDLDVAQYKE YSNYP

UniProtKB: NUXM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #16: NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreduc...

MacromoleculeName: NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 43.349988 KDa
SequenceString: MFRQARFVST FPLKSDINIT KSGKTLLSVG QGGRSSRTGF TATVFGGTGF LGRVVVSKLA KHGTNVVVPF RNDRARRQLK VNGDLGVVN FVEFDIRNLK SIEDSVKYSD IVINLIGTEH FSKNFSIEDV NIGAAERIAK AAKEAGVSRL IHVSSYNANP S SSSDFYAT ...String:
MFRQARFVST FPLKSDINIT KSGKTLLSVG QGGRSSRTGF TATVFGGTGF LGRVVVSKLA KHGTNVVVPF RNDRARRQLK VNGDLGVVN FVEFDIRNLK SIEDSVKYSD IVINLIGTEH FSKNFSIEDV NIGAAERIAK AAKEAGVSRL IHVSSYNANP S SSSDFYAT KGIGEQVVRD NFGPDATIVR PAPMYFRNSP FLNELLQIKA LGGNVIFKKE VYPVHALQVG EAIERIAFDD AT AGQTYEL FGKERYSKKE LREMIKHIIH IGQRGYYPYS AGFYLPAPEP FLYAFALIRQ YISSQPRFNV DQLKRSHINQ EID QSAKTF TDLGMVPDEL SEYLYRYVKP HISYTSQTQN RTVYSKEDIE KLRDYVNTPN DSFNLFNV

UniProtKB: NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 18.241705 KDa
SequenceString:
MLRSLLKVQV RGFSCGRVVS AAKNTNITPA INEGHTDLAK ELVSGAPKEL ITERVARIYQ EAKAATQSGQ HNTETWKIDW DILNKGNRW ENDLMGYQSS SDYMQGTILK FDTREGAIRF AKGQGWDYYI QEPKVRRFVK KDYSANFYHS KGPLKHIRTK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

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Macromolecule #18: NUMM (13 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreduc...

MacromoleculeName: NUMM (13 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 15.717822 KDa
SequenceString:
MLRLTRASIR RISTKELTTA RIKDISKPDE NNLQQQAPNR ISPWSKSQKP RAEAQDNVRF AVKDLAKQPR PYAAIDLIYK QPIRYIEHD NVAVCDGNGG AQGHPKVYIN LDQNKAIPCG YCGLRFAQAA FKEDIESSQN

UniProtKB: NUMM (13 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #19: NI8M (B8) subunit of mitochondrial NADH:ubiquinone oxidoreductase...

MacromoleculeName: NI8M (B8) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 9.858478 KDa
SequenceString:
(ACE)SSKFVFPKV VKELRFFLSQ TGEASVPLRS FLTKTYPAIK KANPTLPILI REAYGIPPSV TARLEKGHEV KTNLEG FSA DQIAKTLQSI A

UniProtKB: NI8M (B8) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #20: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 15.507594 KDa
SequenceString:
MLRNVSRQLL RSSKVYLPQV AVVRTQTPAF VSPSIISKRF NITQSASTLT KEDVLTRSVS VLRGFEVKTK DIDLQTTFVQ DLGMDSLDL NDALVALEEE FDVVFDDKTA NEIKTVGEVV DFVLANYLPT EQSINHTIR

UniProtKB: Acyl carrier protein

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Macromolecule #21: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 14.780592 KDa
SequenceString:
MNRFNHPILR TAFSKSKWNL SKYTSPLFRP SYARTMIPLR TYSSTPSLTK EIIYDRIVEI LESYDNVKQG STISPAASFS KDLGLDSLD TVEVLLELEN EFSILIPDNE ADEIKTVQQA VDYIAAQEDS I

UniProtKB: Acyl carrier protein

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Macromolecule #22: NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductas...

MacromoleculeName: NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 15.590797 KDa
SequenceString:
MRAFTYLRQA QEILIKANKG NPTGLTGLFQ HPSPRPTLIT LYKATLKNLE EKIPKESVYR TAVEAFTKER LSIVENNEII EDIEKKIGS GLVEELIVQA NEEYDLIDKM AEWKAWEELE EKPLDDQWTY FGKKL

UniProtKB: NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #23: BA75_04796T0

MacromoleculeName: BA75_04796T0 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 14.783013 KDa
SequenceString:
MTNPTAFAQT TRYVKDSTEM RTLVLNLYRR YLRHSRGFVN NYNLDIPASQ VRTKIRQEFE RQRFVKDLPL KNVLYMKAQM EFQELVNFW KQQCHVMQYF ESIDHQNKIK GDSFVQKFLK GAQ

UniProtKB: BA75_04796T0

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Macromolecule #24: NADH-ubiquinone oxidoreductase

MacromoleculeName: NADH-ubiquinone oxidoreductase / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 20.718482 KDa
SequenceString:
(ACE)SSTHTNDSL NSSNYVDKLK DTHFVFENDK LPSEIPDVEE VGATTAPLLS AAYFIGAKCQ DYNDDFMLCQ KEAKGN GPI DCLKEGRRVT RCASGVLKDL NTHCSDEFRL HWQCLSFSNL EYKNCRKAES LLNKCVFEKM NLVKKIPGIP EDRQIHL KQ NPVLKPVALH QDSEVAFKKA QNENSI

UniProtKB: NADH-ubiquinone oxidoreductase

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Macromolecule #25: NUJM (B14.7) subunit of mitochondrial NADH:ubiquinone oxidoreduct...

MacromoleculeName: NUJM (B14.7) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 23.507607 KDa
SequenceString: MSETTAEQTV VEQFHLPQPF WPTTKTKEGE YVPKDSIREA SKVFIGSGVL LGGMHFRRLM QYKKGSARPG LAKYLNLFSL SKSQLIGFP VALGAFSFLS HSFANLQEGQ TATGEFAASS IASLIGLSIF RTKKPLSSNV AIAVGMGSLF GFLTWAGNFN L GDNSYRAA ...String:
MSETTAEQTV VEQFHLPQPF WPTTKTKEGE YVPKDSIREA SKVFIGSGVL LGGMHFRRLM QYKKGSARPG LAKYLNLFSL SKSQLIGFP VALGAFSFLS HSFANLQEGQ TATGEFAASS IASLIGLSIF RTKKPLSSNV AIAVGMGSLF GFLTWAGNFN L GDNSYRAA LEAGNGNEGG FDNKVIKTER QGFWEGVYRR PLSQTVEDLG EGRGIITQ

UniProtKB: NUJM (B14.7) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 17.243695 KDa
SequenceString:
(ACE)ATQDLPPIS GYAPIQWKRN LPSRGFRPVI WFLGLCTATG YGFYQISLAN REKIELKREK LWARAHLMPL LQAEQD RNV VRRTFAYYKR EGEIMKDVPW WEVKSTYSNK DIFHPPQTVL FGKHIDRDSG LMYANSRNRF GPDEEETK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

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Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 17.446691 KDa
SequenceString:
MIPFEALIPY AIMFAGFCLG GGVMNSAITA DITKRARGPN QEIKRNVGED EGPRNYTKPR YNTDQWDKYF AVRDLRLTGS LRGQSDNAV AEESFKTNSI QPYSNTRRPW VLRRHIIMKN SPTKWARKYS SRRDEREQEI KDDYIRGMGE H

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

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Macromolecule #28: NI9M (B9) subunit of mitochondrial NADH:ubiquinone oxidoreductase...

MacromoleculeName: NI9M (B9) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 9.181521 KDa
SequenceString:
MAIGDYPVYY KQPIRWLSYH AHTRPHVFYA IAVAALGPVF MMATPLRRKF LYDDHEPLPA NGYPLPNRSR DKTLTGYDD

UniProtKB: NI9M (B9) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #29: BA75_00589T0

MacromoleculeName: BA75_00589T0 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 20.110992 KDa
SequenceString:
(ACE)SGGPVPVFK KYTTGSKGIW EKIRQLLTLV PNRSTGNPLV KNYRVPPPGS QTLKYKDPVT IPAGDIVKNQ YYDRDH RRN YPRLVAFDQT KVSGLLTLGS AQNPRISVGE KGQKELAVFD QSVSLSSTLA TIDPKVVKSE LLAEGGSPVV APSLNKK MK FRLTEESEHG MYPEDYPIRI FAQN

UniProtKB: BA75_00589T0

+
Macromolecule #30: Pichia pastoris NADH-ubiquinone oxidoreductase subunit NEBM

MacromoleculeName: Pichia pastoris NADH-ubiquinone oxidoreductase subunit NEBM
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 9.050411 KDa
SequenceString:
MIASLAGWGL FGVTVRAYQQ GIRKVPFSYY PLGYVYSALG WVAFGYGFNL WTERNDKLLE KRVEKLKESR NLRLSKDD

UniProtKB: Uncharacterized protein

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Macromolecule #31: BA75_05084T0

MacromoleculeName: BA75_05084T0 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 12.251016 KDa
SequenceString:
MAPLQQVGRH GGPNKCFGEF QRVVECMTNA KHDSYEPCNN LKNDYLECRK HKVERYKAFL MQKHAEELGK DPEEIGAPFV RKRRFLTSK TLGLVDGDDY EIGVEKI

UniProtKB: BA75_05084T0

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Macromolecule #32: NUTM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Com...

MacromoleculeName: NUTM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 9.86431 KDa
SequenceString:
MPYLVAFRPA VLVFFFIRQP IGAGLLAGGF LFWQADRHFN FGIRDNIHNF KQTILGFPTQ ASKWETFKQV DAQKRAQLVS GPSEEH

UniProtKB: NUTM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #33: NESM (ESSS) subunit of mitochondrial NADH:ubiquinone oxidoreducta...

MacromoleculeName: NESM (ESSS) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 27.250256 KDa
SequenceString: MYPVIQSAAR RLGVRCFSGT TSVRAFNLQL EKEIRSKIDS LPKLNNIFQN ANGSVRQPSD TELQTIAELN SLVTNRKLSL TDYLFQNAA NQALYVDNEA DLKELDISSR QVVDKVPFKD AKTGEIKWKV VRENENEGWE KIMYVGFVPT MILIFAIVLF K DDKSIDEW ...String:
MYPVIQSAAR RLGVRCFSGT TSVRAFNLQL EKEIRSKIDS LPKLNNIFQN ANGSVRQPSD TELQTIAELN SLVTNRKLSL TDYLFQNAA NQALYVDNEA DLKELDISSR QVVDKVPFKD AKTGEIKWKV VRENENEGWE KIMYVGFVPT MILIFAIVLF K DDKSIDEW AMDELLLRLK EKNLSDVELI DELDKLSKSH VGKIGASEIN KRDAVIIERI LNGEYDQLSG LKKKELPIID L

UniProtKB: NESM (ESSS) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #34: NUSM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Com...

MacromoleculeName: NUSM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 21.224576 KDa
SequenceString:
MLTRRSVGLL QSTRISPVLT NFSSSRFYSA HHESDKKHDD HHEEHHDEHH DDHHTPLGPA ENIFNKWNVT PFVWLFATVG IFQWDKSHR ESHDGKGILD FLGEIKVKES LDTYFKEYQT KVASTADLYG LISFTPKRDI HHYVIRVDEV PGKHFPTNTT G NFGNTVDF ENLEPRRKPI NPYL

UniProtKB: NUSM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #35: NUUM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Com...

MacromoleculeName: NUUM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 8.335509 KDa
SequenceString:
MSGHHIHTPS YIKPPTGGWN FTPANWKQNT VLVLVGFTAC CVGLYKLGEA GSYNPKLKYK EEELSRWNQS AKKN

UniProtKB: NUUM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #36: Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 7.071344 KDa
SequenceString:
MSAHHAFKYV RGAIVPKPKV HPGYVITSKF LGGLMWFWIF YRAKQDYPVW FGLKHPWEH

UniProtKB: Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #37: NB2M (B12) subunit of mitochondrial NADH:ubiquinone oxidoreductas...

MacromoleculeName: NB2M (B12) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 7.141992 KDa
SequenceString:
MAGNPWKRRD AWRSQGAFTR ANRFRGALPG FFWGLGAFGV YCVYEKVFLT NDHHGHHEEH H

UniProtKB: NB2M (B12) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #38: NIAM (ASHI) subunit of mitochondrial NADH:ubiquinone oxidoreducta...

MacromoleculeName: NIAM (ASHI) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 17.944211 KDa
SequenceString:
MLRQAYRLAG KTNSITSSSV FIRNKSFLPH FIPKNYSYGD YPRLKPENLQ KRDPYLKYDD QANKRNFNEP LHPQQDDLDM WAPDHFDFV QDSIALKWFG TFFGVLAVFS SVIYIGGLYP EKPANPRNYP FSGLAKDLGA SSEEDTLLYA AKIDKSI

UniProtKB: NIAM (ASHI) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

+
Macromolecule #39: NB5M (B15) subunit of mitochondrial NADH:ubiquinone oxidoreductas...

MacromoleculeName: NB5M (B15) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 9.437788 KDa
SequenceString:
MAGHGPHLLR EDASFQRYGQ LRDNLATYWK FNRKAARNVF IFLVAVPAGL GYLAYNYEGK TPFVGKRRTE PIFEQDYVPR K

UniProtKB: NB5M (B15) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

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Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 12.845718 KDa
SequenceString:
MSSIARKAVV PFSEANKKYV SSLYRKSLRT AHDWINKTEF YRVKASEIRS RFDANKHIND PNELKVIFKE TEALLEDYAH PDPIIPPCR PGGSKFERNI PPRVEARIPI DV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

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Macromolecule #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 10.473039 KDa
SequenceString:
MGVSELPELL SHEEMKQNKI PLAYRDRCAR LLVPLNKCRK EGWYMPWNCV QERHDYEQCQ YLDFKRRVKE LEEIKKQRDA ADASADL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

+
Macromolecule #42: NIDM (PDSW) subunit of mitochondrial NADH:ubiquinone oxidoreducta...

MacromoleculeName: NIDM (PDSW) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 10.787037 KDa
SequenceString:
MVDHVRSPNV SFDDIDYNNS QDLHNAQESL LREQWIRVSA LKVCRKALES CYKTSGPNHY EDCREIAEKY LNMLPDHRIQ GFLGYQKND PSK

UniProtKB: NIDM (PDSW) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I)

+
Macromolecule #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit
type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Komagataella pastoris (fungus)
Molecular weightTheoretical: 16.331366 KDa
SequenceString:
(ACE)SSSIQRTLR NLWKNGFKKS FKQLQGYNDI KSGRLVGTDS FGNKFYEVDD PVEIHMRTRF VEYTGFWRQD MSEVEP GWH YWLAYGTDVP PNQLKPEEKS VRAYPVKKHV PNLTYTDGAY VPYNTARPKC ATWDPKVTER P

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit

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Macromolecule #44: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 44 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #45: DIUNDECYL PHOSPHATIDYL CHOLINE

MacromoleculeName: DIUNDECYL PHOSPHATIDYL CHOLINE / type: ligand / ID: 45 / Number of copies: 21 / Formula: PLC
Molecular weightTheoretical: 622.834 Da
Chemical component information

ChemComp-PLC:
DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipid*YM

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Macromolecule #46: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 46 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

+
Macromolecule #47: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 47 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

+
Macromolecule #48: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 48 / Number of copies: 1 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #49: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 49 / Number of copies: 19 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

+
Macromolecule #50: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 50 / Number of copies: 4 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

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Macromolecule #51: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 51 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

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Macromolecule #52: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 52 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #53: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butan...

MacromoleculeName: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate
type: ligand / ID: 53 / Number of copies: 2 / Formula: EHZ
Molecular weightTheoretical: 584.703 Da
Chemical component information

ChemComp-EHZ:
~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.7 mg/mL
BufferpH: 7.5
Component:
ConcentrationNameFormula
10.0 mMMOPS
50.0 mMpotassium chlorideKCl

Details: 10 mM MOPS, 50 mM KCl, pH 7.5 at 4 degrees celsius.
GridModel: Quantifoil R0.6/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 180 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa
Details: Glow discharge 90s 20mA + chloroform and ethanol clean. Glow discharge 2X 90s 20mA, flipping in between.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK III / Details: 2s blot time and -10 blot force.
DetailsLMNG-solubilised Pichia Pastoris CI reconstituted in to nanodiscs (8:1:1 ratio of DOPC:DOPE:CDL [0.5 mg total] and 20 nM final Q10)

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 7574 / Average exposure time: 5.85 sec. / Average electron dose: 47.79 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 1350752
CTF correctionType: PHASE FLIPPING ONLY
Startup modelType of model: INSILICO MODEL
In silico model: Initial model generated from 3D initial model job in CryoSPARC v3.3.2
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.34 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 25125
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 4 / Avg.num./class: 40000
Details: Not an even split in final numbers for the classes: class 1 53,716; class 2 61,256; class 3 25,125; class 4 18,608. Final classification used focused maps to target the compositionally heterogenous regions.
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

9ihr


Chain - Source name: PDB / Chain - Initial model type: experimental model / Details: taken from the state 1 model
DetailsThe initial model was built into a map using ModelAngelo in RELION v5.0 for state 1. Appropriate subunits were deleted for this state before being manually inspected in Coot and final refinements with ligand restraints imposed performed in PHENIX v.1.21_5207
RefinementSpace: REAL
Output model

PDB-9ihp:
Open state without NUQM and with flavoprotein (classification state 3) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs

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