EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Single-stranded DNA drives σ subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 53, Issue 7, Year 2025
掲載日	2025年4月10日
著者	Rishi Kishore Vishwakarma / Nils Marechal / Zakia Morichaud / Mickaël Blaise / Emmanuel Margeat / Konstantin Brodolin /
PubMed 要旨	Initiation of transcription requires the formation of the "open" promoter complex (RPo). For this, the σ subunit of bacterial RNA polymerase (RNAP) binds to the nontemplate strand of the -10 element ...Initiation of transcription requires the formation of the "open" promoter complex (RPo). For this, the σ subunit of bacterial RNA polymerase (RNAP) binds to the nontemplate strand of the -10 element sequence of promoters and nucleates DNA unwinding. This is accompanied by a cascade of conformational changes on RNAP, the exact mechanics of which remains elusive. Here, using single-molecule Förster resonance energy transfer and cryo-electron microscopy, we explored the conformational landscape of RNAP from the human pathogen Mycobacterium tuberculosis upon binding to a single-stranded DNA (ssDNA) fragment that includes the -10 element sequence (-10 ssDNA). We found that like the transcription activator RNAP-binding protein A, -10 ssDNA induced σ subunit loading onto the DNA/RNA channels of RNAP. This triggered RNAP clamp closure and unswiveling that are required for RPo formation and RNA synthesis initiation. Our results reveal a mechanism of ssDNA-guided RNAP maturation and identify the σ subunit as a regulator of RNAP conformational dynamics.
リンク	Nucleic Acids Res / PubMed:40240004 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 4.33 Å
構造データ	50508 9fjp EMDB-50508, PDB-9fjp: Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-50509: cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - consensus map 手法: EM (単粒子) / 解像度: 3.33 Å 50510 9fjr EMDB-50510, PDB-9fjr: Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - sigma-B docked conformation 手法: EM (単粒子) / 解像度: 3.43 Å 50511 9fjs EMDB-50511: Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - sigma-B udocked conformation PDB-9fjs: Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - sigma-B undocked conformation 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-50512: Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - swiveled conformation 手法: EM (単粒子) / 解像度: 4.33 Å EMDB-50514: Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - unswiveled conformation 手法: EM (単粒子) / 解像度: 3.79 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	mycobacterium tuberculosis h37rv (結核菌) escherichia coli (大腸菌)
キーワード	TRANSCRIPTION / RNA polymerase; sigma factor; SigB / stress response; -10 promoter element; promoter recognition; tuberculosis