EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM snapshots of NMDA receptor activation illuminate sequential rearrangements.
ジャーナル・号・ページ	Sci Adv, Vol. 11, Issue 39, Page eadx4647, Year 2025
掲載日	2025年9月26日
著者	Jamie A Abbott / Junhoe Kim / Beiying Liu / Gabriela K Popescu / Eric Gouaux / Farzad Jalali-Yazdi /
PubMed 要旨	Canonical -methyl-d-aspartate receptors (NMDARs) are glutamate-gated ion channels with critical roles in the development and function of the nervous system. The excitatory currents they produce ...Canonical -methyl-d-aspartate receptors (NMDARs) are glutamate-gated ion channels with critical roles in the development and function of the nervous system. The excitatory currents they produce reflect stochastic transitions between multiple agonist-bound closed- and open-pore states. We leveraged the intrinsically high open probability () of NMDARs composed of GluN1 and GluN2A subunits, together with judiciously chosen mutants and ligands, to achieve conditions in which receptors had a near unity. Using single-particle cryo-electron microscopy (cryo-EM), we captured three activated receptor states, each with distinct conformations of the gate-forming M3 helices. Separately, we carried out single-channel electrophysiology, together with statistical modeling, to relate the cryo-EM structures to the gating reaction. NMDAR channel opening involves bending of the pore-forming M3 helices to produce a transient open-channel conformation, subsequently stabilized by new interactions between the D2-M3 linkers with the pre-M1 helices and the pre-M4 loops, to yield the stable open channel.
リンク	Sci Adv / PubMed:40991709 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.61 Å
構造データ	45279 9c7c 9c7e EMDB-45279, PDB-9c7c: Diheteromeric GluN1/GluN2A (delM653) in nanodisc complexed with glycine, glutamate, and GNE-4123, open conformation PDB-9c7e: Diheteromeric GluN1/GluN2A (delM653) in nanodisc complex with glycine, glutamate, and GNE-4123, open conformation, C2 symmetry 手法: EM (単粒子) / 解像度: 3.1 Å 45283 9c7p EMDB-45283, PDB-9c7p: Diheteromeric GluN1/GluN2A (delM653) in digitonin complexed with glycine, glutamate, and GNE-4123 手法: EM (単粒子) / 解像度: 4.61 Å 45284 9c7q EMDB-45284: Diheteromeric GluN1/GluN2A (delM653) in digitonin complexed with glycine and glutamate PDB-9c7q: Diheteromeric NMDA receptor GluN1/GluN2A, in complex with glycine and glutamate 手法: EM (単粒子) / 解像度: 4.05 Å 45285 9c7r EMDB-45285, PDB-9c7r: Diheteromeric GluN1/GluN2A (M817V) in digitonin complexed with glycine, glutamate, and GNE-4123 手法: EM (単粒子) / 解像度: 3.99 Å
化合物	PDB-1auv: STRUCTURE OF THE C DOMAIN OF SYNAPSIN IA FROM BOVINE BRAIN ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-HP6: HEPTANE ChemComp-Y01: CHOLESTEROL HEMISUCCINATE ChemComp-GLY: GLYCINE ChemComp-D12: DODECANE ChemComp-GLU: GLUTAMIC ACID ChemComp-D10: DECANE ChemComp-HEX: HEXANE
由来	rattus norvegicus (ドブネズミ) aequorea victoria (オワンクラゲ)
キーワード	TRANSPORT PROTEIN / ion channel / NMDA / positive allosteric modulator / open pore conformation / pre-open pore conformation