EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Dimerization and antidepressant recognition at noradrenaline transporter.
ジャーナル・号・ページ	Nature, Vol. 630, Issue 8015, Page 247-254, Year 2024
掲載日	2024年5月15日
著者	Heng Zhang / Yu-Ling Yin / Antao Dai / Tianwei Zhang / Chao Zhang / Canrong Wu / Wen Hu / Xinheng He / Benxun Pan / Sanshan Jin / Qingning Yuan / Ming-Wei Wang / Dehua Yang / H Eric Xu / Yi Jiang /
PubMed 要旨	The noradrenaline transporter has a pivotal role in regulating neurotransmitter balance and is crucial for normal physiology and neurobiology. Dysfunction of noradrenaline transporter has been ...The noradrenaline transporter has a pivotal role in regulating neurotransmitter balance and is crucial for normal physiology and neurobiology. Dysfunction of noradrenaline transporter has been implicated in numerous neuropsychiatric diseases, including depression and attention deficit hyperactivity disorder. Here we report cryo-electron microscopy structures of noradrenaline transporter in apo and substrate-bound forms, and as complexes with six antidepressants. The structures reveal a noradrenaline transporter dimer interface that is mediated predominantly by cholesterol and lipid molecules. The substrate noradrenaline binds deep in the central binding pocket, and its amine group interacts with a conserved aspartate residue. Our structures also provide insight into antidepressant recognition and monoamine transporter selectivity. Together, these findings advance our understanding of noradrenaline transporter regulation and inhibition, and provide templates for designing improved antidepressants to treat neuropsychiatric disorders.
リンク	Nature / PubMed:38750358
手法	EM (単粒子)
解像度	2.89 - 3.29 Å
構造データ	39064 8y8z EMDB-39064, PDB-8y8z: Structure of NET-Maprotiline in outward-open state 手法: EM (単粒子) / 解像度: 3.29 Å 39065 8y90 EMDB-39065, PDB-8y90: Structure of NET-Nefopam in outward-open state 手法: EM (単粒子) / 解像度: 3.15 Å 39066 8y91 EMDB-39066, PDB-8y91: Structure of NET-nomifensine in outward-open state 手法: EM (単粒子) / 解像度: 3.13 Å 39067 8y92 EMDB-39067, PDB-8y92: structure of NET-Atomoxetine in outward-open state 手法: EM (単粒子) / 解像度: 3.29 Å 39068 8y93 EMDB-39068, PDB-8y93: Structure of NET-Amitriptyline in outward-open state 手法: EM (単粒子) / 解像度: 3.0 Å 39069 8y94 EMDB-39069, PDB-8y94: Structure of Apo human norepinephrine transporter NET 手法: EM (単粒子) / 解像度: 3.29 Å 39070 8y95 EMDB-39070, PDB-8y95: Structure of NET-NE in Occluded state 手法: EM (単粒子) / 解像度: 3.24 Å 39533 8yr2 EMDB-39533, PDB-8yr2: Structure of NET-Nisoxetine in outward-open state 手法: EM (単粒子) / 解像度: 2.89 Å
化合物	PDB-1lx3: Unknown entry ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-CL: Unknown entry / クロリド ChemComp-HOH: WATER PDB-1lx6: Crystal Structure of E. Coli Enoyl Reductase-NAD+ with a Bound Benzamide Inhibitor PDB-1lx5: Crystal Structure of the BMP7/ActRII Extracellular Domain Complex PDB 未公開エントリ PDB-1lx4: Solution NMR Structure Of E. coli N-Ada/DNA transcription complex ChemComp-TP0: Amitriptyline / アミトリプチリン / 抗うつ薬YM ChemComp-E5E: Noradrenaline / (R)-2-(3,4-ジヒドロキシフェニル)-2-ヒドロキシエタンアミニウム / 神経伝達物質, ホルモンYM ChemComp-41U: nisoxetine / (S)-ニソキセチン / 抗うつ薬, 阻害剤YM ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate / 1-O-(1-O,2-O-ジオクタノイル-L-グリセロ-3-ホスホ)-D-myo-イノシト-ル4,5-ビスりん酸 ChemComp-CLR: CHOLESTEROL / コレステロ-ル PDB-1lx7*: Structure of E. coli uridine phosphorylase at 2.0A
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN/INHIBITOR / transporter / TRANSPORT PROTEIN-INHIBITOR COMPLEX / transpoter / TRANSPORT PROTEIN / neurotransmitter / transporters / substrate