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-Structure paper
タイトル | Structure and function of the ABC transporter ABCB19 in brassinosteroid export. |
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ジャーナル・号・ページ | Science, Vol. 383, Issue 6689, Page eadj4591, Year 2024 |
掲載日 | 2024年3月22日 |
著者 | Wei Ying / Yaowei Wang / Hong Wei / Yongming Luo / Qian Ma / Heyuan Zhu / Hilde Janssens / Nemanja Vukašinović / Miroslav Kvasnica / Johan M Winne / Yongxiang Gao / Shutang Tan / Jiří Friml / Xin Liu / Eugenia Russinova / Linfeng Sun / |
PubMed 要旨 | Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but ...Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In , ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling. |
リンク | Science / PubMed:38513023 |
手法 | EM (単粒子) |
解像度 | 3.4 - 3.9 Å |
構造データ | EMDB-37690, PDB-8woi: EMDB-37692, PDB-8wom: EMDB-37694, PDB-8woo: EMDB-37705, PDB-8wp0: |
化合物 | ChemComp-BLD: ChemComp-MG: ChemComp-ANP: ChemComp-ATP: |
由来 |
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キーワード | TRANSPORT PROTEIN / ABC transporter / wild-type / apo state / brassinolide-bound state |