EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 3, Page 424-435, Year 2024
掲載日	2024年1月4日
著者	Kendra Marcus / Yongjian Huang / Subu Subramanian / Christine L Gee / Kent Gorday / Sam Ghaffari-Kashani / Xiao Ran Luo / Lisa Zheng / Michael O'Donnell / Sriram Subramaniam / John Kuriyan /
PubMed 要旨	Clamp loaders are AAA+ ATPases that facilitate high-speed DNA replication. In eukaryotic and bacteriophage clamp loaders, ATP hydrolysis requires interactions between aspartate residues in one ...Clamp loaders are AAA+ ATPases that facilitate high-speed DNA replication. In eukaryotic and bacteriophage clamp loaders, ATP hydrolysis requires interactions between aspartate residues in one protomer, present in conserved 'DEAD-box' motifs, and arginine residues in adjacent protomers. We show that functional defects resulting from a DEAD-box mutation in the T4 bacteriophage clamp loader can be compensated by widely distributed single mutations in the ATPase domain. Using cryo-EM, we discovered an unsuspected inactive conformation of the clamp loader, in which DNA binding is blocked and the catalytic sites are disassembled. Mutations that restore function map to regions of conformational change upon activation, suggesting that these mutations may increase DNA affinity by altering the energetic balance between inactive and active states. Our results show that there are extensive opportunities for evolution to improve catalytic efficiency when an inactive intermediate is involved.
リンク	Nat Struct Mol Biol / PubMed:38177685 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.628 - 3.21 Å
構造データ	42399 8unf EMDB-42399, PDB-8unf: Cryo-EM structure of T4 Bacteriophage Clamp Loader with Sliding Clamp and DNA 手法: EM (単粒子) / 解像度: 3.15 Å 42402 8unh EMDB-42402, PDB-8unh: Cryo-EM structure of T4 Bacteriophage Clamp Loader with Sliding Clamp 手法: EM (単粒子) / 解像度: 3.21 Å PDB-8uh7: Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog 手法: X-RAY DIFFRACTION / 解像度: 2.628 Å PDB-8uk9: Structure of T4 Bacteriophage clamp loader mutant D110C bound to the T4 clamp, primer-template DNA, and ATP analog 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å
化合物	ChemComp-08T: [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-AF3: ALUMINUM FLUORIDE / トリフルオロアルミニウム ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM
由来	tequatrovirus t4 (ウイルス) tequatrovirus (ウイルス)
キーワード	REPLICATION/DNA / Clamp loader / T4 / AAA+ATPase / REPLICATION / REPLICATION-DNA complex / DNA BINDING PROTEIN/DNA / DNA Replication / AAA+ ATPase / Bacteriophage / Complex / DNA / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / active / DNA-bound / autoinhibited / DNA-free / catalytically inactive / stable