+検索条件
-Structure paper
タイトル | Structures of influenza A and B replication complexes give insight into avian to human host adaptation and reveal a role of ANP32 as an electrostatic chaperone for the apo-polymerase. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 6910, Year 2024 |
掲載日 | 2024年8月19日 |
著者 | Benoît Arragain / Tim Krischuns / Martin Pelosse / Petra Drncova / Martin Blackledge / Nadia Naffakh / Stephen Cusack / |
PubMed 要旨 | Replication of influenza viral RNA depends on at least two viral polymerases, a parental replicase and an encapsidase, and cellular factor ANP32. ANP32 comprises an LRR domain and a long C-terminal ...Replication of influenza viral RNA depends on at least two viral polymerases, a parental replicase and an encapsidase, and cellular factor ANP32. ANP32 comprises an LRR domain and a long C-terminal low complexity acidic region (LCAR). Here we present evidence suggesting that ANP32 is recruited to the replication complex as an electrostatic chaperone that stabilises the encapsidase moiety within apo-polymerase symmetric dimers that are distinct for influenza A and B polymerases. The ANP32 bound encapsidase, then forms the asymmetric replication complex with the replicase, which is embedded in a parental ribonucleoprotein particle (RNP). Cryo-EM structures reveal the architecture of the influenza A and B replication complexes and the likely trajectory of the nascent RNA product into the encapsidase. The cryo-EM map of the FluB replication complex shows extra density attributable to the ANP32 LCAR wrapping around and stabilising the apo-encapsidase conformation. These structures give new insight into the various mutations that adapt avian strain polymerases to use the distinct ANP32 in mammalian cells. |
リンク | Nat Commun / PubMed:39160148 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.54 - 3.64 Å |
構造データ | EMDB-19366, PDB-8rmp: EMDB-19367, PDB-8rmq: EMDB-19368, PDB-8rmr: EMDB-19369, PDB-8rms: EMDB-19382, PDB-8rn0: EMDB-19383, PDB-8rn1: EMDB-19384, PDB-8rn2: EMDB-19385, PDB-8rn3: EMDB-19386, PDB-8rn4: EMDB-19387, PDB-8rn5: EMDB-19388, PDB-8rn6: EMDB-19389, PDB-8rn7: EMDB-19390, PDB-8rn8: EMDB-19391, PDB-8rn9: EMDB-19392, PDB-8rna: EMDB-19393, PDB-8rnb: EMDB-19394, PDB-8rnc: |
化合物 | ChemComp-MG: ChemComp-HOH: |
由来 |
|
キーワード | VIRAL PROTEIN / Viral polymerase |