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-Structure paper
タイトル | Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 1844, Year 2024 |
掲載日 | 2024年2月28日 |
著者 | Jaroslaw Sedzicki / Dongchun Ni / Frank Lehmann / Henning Stahlberg / Christoph Dehio / |
PubMed 要旨 | The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major ...The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). CβG is produced by the cyclic glucan synthase (Cgs), a multi-domain membrane protein. So far, its structure as well as the mechanism underlining the synthesis have not been clarified. Here we use cryo-electron microscopy (cryo-EM) and functional approaches to study Cgs from A. tumefaciens. We determine the structure of this complex protein machinery and clarify key aspects of CβG synthesis, revealing a distinct mechanism that uses a tyrosine-linked oligosaccharide intermediate in cycles of polymerization and processing of the glucan chain. Our research opens possibilities for combating pathogens that rely on polysaccharide virulence factors and may lead to synthetic biology approaches for producing complex cyclic sugars. |
リンク | Nat Commun / PubMed:38418509 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.28 - 5.99 Å |
構造データ | EMDB-19114, PDB-8rf0: EMDB-19116, PDB-8rf9: EMDB-19118, PDB-8rfe: EMDB-19119, PDB-8rfg: EMDB-19123: cyclic b-1,2-glucan synthase IGT mutant |
化合物 | ChemComp-UPG: ChemComp-HOH: |
由来 |
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キーワード | ANTIBIOTIC / cgs / cyclic glucan |