EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	GTPBP8 plays a role in mitoribosome formation in human mitochondria.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 5664, Year 2024
掲載日	2024年7月5日
著者	Miriam Cipullo / Genís Valentín Gesé / Shreekara Gopalakrishna / Annika Krueger / Vivian Lobo / Maria A Pirozhkova / James Marks / Petra Páleníková / Dmitrii Shiriaev / Yong Liu / Jelena Misic / Yu Cai / Minh Duc Nguyen / Abubakar Abdelbagi / Xinping Li / Michal Minczuk / Markus Hafner / Daniel Benhalevy / Aishe A Sarshad / Ilian Atanassov / B Martin Hällberg / Joanna Rorbach /
PubMed 要旨	Mitochondrial gene expression relies on mitoribosomes to translate mitochondrial mRNAs. The biogenesis of mitoribosomes is an intricate process involving multiple assembly factors. Among these ...Mitochondrial gene expression relies on mitoribosomes to translate mitochondrial mRNAs. The biogenesis of mitoribosomes is an intricate process involving multiple assembly factors. Among these factors, GTP-binding proteins (GTPBPs) play important roles. In bacterial systems, numerous GTPBPs are required for ribosome subunit maturation, with EngB being a GTPBP involved in the ribosomal large subunit assembly. In this study, we focus on exploring the function of GTPBP8, the human homolog of EngB. We find that ablation of GTPBP8 leads to the inhibition of mitochondrial translation, resulting in significant impairment of oxidative phosphorylation. Structural analysis of mitoribosomes from GTPBP8 knock-out cells shows the accumulation of mitoribosomal large subunit assembly intermediates that are incapable of forming functional monosomes. Furthermore, fPAR-CLIP analysis reveals that GTPBP8 is an RNA-binding protein that interacts specifically with the mitochondrial ribosome large subunit 16 S rRNA. Our study highlights the role of GTPBP8 as a component of the mitochondrial gene expression machinery involved in mitochondrial large subunit maturation.
リンク	Nat Commun / PubMed:38969660 / PubMed Central
手法	EM (単粒子)
解像度	2.42 - 6.75 Å
構造データ	18438 8qrk EMDB-18438, PDB-8qrk: mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 1 手法: EM (単粒子) / 解像度: 6.75 Å 18439 8qrl EMDB-18439, PDB-8qrl: mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 2 手法: EM (単粒子) / 解像度: 3.96 Å 18440 8qrm EMDB-18440, PDB-8qrm: mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 3 手法: EM (単粒子) / 解像度: 3.05 Å 18443 8qrn EMDB-18443: mt-SSU assembly intermediate in GTPBP8 knock-out cells, state 4 PDB-8qrn: mt-SSU in GTPBP8 knock-out cells, state 4 手法: EM (単粒子) / 解像度: 2.98 Å 18460 8qu1 EMDB-18460, PDB-8qu1: mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 1 手法: EM (単粒子) / 解像度: 2.74 Å 18461 8qu5 EMDB-18461, PDB-8qu5: mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 2 手法: EM (単粒子) / 解像度: 2.42 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM ChemComp-SPM: SPERMINE / スペルミン ChemComp-SRY: STREPTOMYCIN / ストレプトマイシン / 薬剤, 抗生剤YM ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン ChemComp-HOH: WATER ChemComp-FME: N-FORMYLMETHIONINE / N-ホルミルメチオニン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / Mitochondria / Assembly / GTPBP8