EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of tRNA recognition by the mC RNA methyltransferase METTL6 in complex with SerRS seryl-tRNA synthetase.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 10, Page 1614-1624, Year 2024
掲載日	2024年6月25日
著者	Philipp Throll / Luciano G Dolce / Palma Rico-Lastres / Katharina Arnold / Laura Tengo / Shibom Basu / Stefanie Kaiser / Robert Schneider / Eva Kowalinski /
PubMed 要旨	Methylation of cytosine 32 in the anticodon loop of tRNAs to 3-methylcytosine (mC) is crucial for cellular translation fidelity. Misregulation of the RNA methyltransferases setting this modification ...Methylation of cytosine 32 in the anticodon loop of tRNAs to 3-methylcytosine (mC) is crucial for cellular translation fidelity. Misregulation of the RNA methyltransferases setting this modification can cause aggressive cancers and metabolic disturbances. Here, we report the cryo-electron microscopy structure of the human mC tRNA methyltransferase METTL6 in complex with seryl-tRNA synthetase (SerRS) and their common substrate tRNA. Through the complex structure, we identify the tRNA-binding domain of METTL6. We show that SerRS acts as the tRNA substrate selection factor for METTL6. We demonstrate that SerRS augments the methylation activity of METTL6 and that direct contacts between METTL6 and SerRS are necessary for efficient tRNA methylation. Finally, on the basis of the structure of METTL6 in complex with SerRS and tRNA, we postulate a universal tRNA-binding mode for mC RNA methyltransferases, including METTL2 and METTL8, suggesting that these mammalian paralogs use similar ways to engage their respective tRNA substrates and cofactors.
リンク	Nat Struct Mol Biol / PubMed:38918637 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.42 - 5.6 Å
構造データ	17528 8p7b EMDB-17528, PDB-8p7b: CryoEM structure of METTL6 tRNA SerRS complex in a 1:2:2 stoichiometry 手法: EM (単粒子) / 解像度: 2.42 Å 17529 8p7c EMDB-17529, PDB-8p7c: CryoEM structure of METTL6 tRNA SerRS complex in a 2:2:2 stoichiometry 手法: EM (単粒子) / 解像度: 3.7 Å 17530 8p7d EMDB-17530, PDB-8p7d: CryoEM structure of METTL6 tRNA SerRS complex in a 1:1:2 stoichiometry 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-17531: SerRS bound to serine tRNA 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-17532: Serine tRNA from Trichoplusia ni 手法: EM (単粒子) / 解像度: 5.6 Å PDB-8owx: Crystal Structure of METTL6 bound to SAH 手法: X-RAY DIFFRACTION / 解像度: 2.601 Å PDB-8owy: Crystal structure of METTL6 mutant 40-269 bound to SAH 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å
化合物	ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン ChemComp-B3P: 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / 2,2′-(トリメチレンビスイミノ)ビス[2-(ヒドロキシメチル)プロパン-1,3-ジオ-ル] / pH緩衝剤YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH*: WATER
由来	homo sapiens (ヒト) trichoplusia ni (イラクサキンウワバ)
キーワード	RNA BINDING PROTEIN / tRNA modification / m3C / methyltransferase / METTL6 / tRNA / SerRS / Serine tRNA / 3-Methylcytosine