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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Serine tRNA from Trichoplusia ni | |||||||||
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![]() | Serine tRNA / RNA BINDING PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 5.6 Å | |||||||||
![]() | Throll P / Dolce LG / Kowalinski E | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structural basis of the cooperation between the m3C-RNA-methyltransferase METTL6 with SerRS amino-acyl-synthetase for tRNA recognition Authors: Throll P / Dolce LG / Lastres PR / Arnold K / Tengo L / Basu S / Kaiser S / Schneider R / Kowalinski E | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 117 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12 KB 12 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.3 KB | Display | ![]() |
Images | ![]() | 16.2 KB | ||
Masks | ![]() | 244.1 MB | ![]() | |
Filedesc metadata | ![]() | 4.3 KB | ||
Others | ![]() ![]() | 226.6 MB 226.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 647 KB | Display | ![]() |
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Full document | ![]() | 646.6 KB | Display | |
Data in XML | ![]() | 22.4 KB | Display | |
Data in CIF | ![]() | 28.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.645 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_17532_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_17532_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : METTL6 tRNA SerRS complex in a 1:2:2 stoichiometry
Entire | Name: METTL6 tRNA SerRS complex in a 1:2:2 stoichiometry |
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Components |
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-Supramolecule #1: METTL6 tRNA SerRS complex in a 1:2:2 stoichiometry
Supramolecule | Name: METTL6 tRNA SerRS complex in a 1:2:2 stoichiometry / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Serine tRNA
Macromolecule | Name: Serine tRNA / type: rna / ID: 1 |
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Source (natural) | Organism: ![]() |
Sequence | String: GCAGUGGUGG C(4AC)GAGU(OMG)G(H2U)U AAGGC(M2G)UCGG A(JMH)UUGA(6IA)A(PSU)C CGA(OMU)UCGCUC UGCGAG(5MC)GUG GG(5MU)(PSU)CG(1MA)AUC CCACCCACUG CGCCA |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 63.27 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |