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-Structure paper
タイトル | Structure of a fungal 1,3-β-glucan synthase. |
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ジャーナル・号・ページ | Sci Adv, Vol. 9, Issue 37, Page eadh7820, Year 2023 |
掲載日 | 2023年9月15日 |
![]() | Chao-Ran Zhao / Zi-Long You / Dan-Dan Chen / Jing Hang / Zhao-Bin Wang / Meng Ji / Le-Xuan Wang / Peng Zhao / Jie Qiao / Cai-Hong Yun / Lin Bai / ![]() |
PubMed 要旨 | 1,3-β-Glucan serves as the primary component of the fungal cell wall and is produced by 1,3-β-glucan synthase located in the plasma membrane. This synthase is a molecular target for antifungal ...1,3-β-Glucan serves as the primary component of the fungal cell wall and is produced by 1,3-β-glucan synthase located in the plasma membrane. This synthase is a molecular target for antifungal drugs such as echinocandins and the triterpenoid ibrexafungerp. In this study, we present the cryo-electron microscopy structure of 1,3-β-glucan synthase (Fks1) at 2.47-Å resolution. The structure reveals a central catalytic region adopting a cellulose synthase fold with a cytosolic conserved GT-A-type glycosyltransferase domain and a closed transmembrane channel responsible for glucan transportation. Two extracellular disulfide bonds are found to be crucial for Fks1 enzymatic activity. Through structural comparative analysis with cellulose synthases and structure-guided mutagenesis studies, we gain previously unknown insights into the molecular mechanisms of fungal 1,3-β-glucan synthase. |
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手法 | EM (単粒子) |
解像度 | 2.47 Å |
構造データ | EMDB-36748, PDB-8jzn: |
化合物 | ![]() ChemComp-POV: ![]() ChemComp-AV0: ![]() ChemComp-ERG: |
由来 |
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![]() | TRANSFERASE / fungi / drug target |