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-Structure paper
タイトル | Structure of the transcription open complex of distinct σ factors. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 6455, Year 2023 |
掲載日 | 2023年10月13日 |
![]() | Jie Li / Haonan Zhang / Dongyu Li / Ya-Jun Liu / Edward A Bayer / Qiu Cui / Yingang Feng / Ping Zhu / ![]() ![]() |
PubMed 要旨 | Bacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ ...Bacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ paralogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σ factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium, Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and binding to promoter DNA. The key characteristics that determine the specificities of the σ paralogues were further revealed by comparison of the two structures. Consequently, the σ factors represent a distinct set of the σ-family σ factors, thus highlighting the diversity of bacterial transcription. |
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手法 | EM (単粒子) |
解像度 | 3.03 - 3.36 Å |
構造データ | EMDB-35130, PDB-8i23: EMDB-35131, PDB-8i24: |
化合物 | ![]() ChemComp-ZN: ![]() ChemComp-MG: |
由来 |
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![]() | TRANSCRIPTION/DNA / TRANSCRIPTION OPEN COMPLEX / SIGI / TRANSCRIPTION / TRANSCRIPTION-DNA complex |