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-Structure paper
タイトル | Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 2050, Year 2023 |
掲載日 | 2023年4月12日 |
著者 | Zhennan Zhao / Youhua Huang / Congcong Liu / Dongjie Zhu / Shuaixin Gao / Sheng Liu / Ruchao Peng / Ya Zhang / Xiaohong Huang / Jianxun Qi / Catherine C L Wong / Xinzheng Zhang / Peiyi Wang / Qiwei Qin / George F Gao / |
PubMed 要旨 | Singapore grouper iridovirus (SGIV), one of the nucleocytoviricota viruses (NCVs), is a highly pathogenic iridovirid. SGIV infection results in massive economic losses to the aquaculture industry and ...Singapore grouper iridovirus (SGIV), one of the nucleocytoviricota viruses (NCVs), is a highly pathogenic iridovirid. SGIV infection results in massive economic losses to the aquaculture industry and significantly threatens global biodiversity. In recent years, high morbidity and mortality in aquatic animals have been caused by iridovirid infections worldwide. Effective control and prevention strategies are urgently needed. Here, we present a near-atomic architecture of the SGIV capsid and identify eight types of capsid proteins. The viral inner membrane-integrated anchor protein colocalizes with the endoplasmic reticulum (ER), supporting the hypothesis that the biogenesis of the inner membrane is associated with the ER. Additionally, immunofluorescence assays indicate minor capsid proteins (mCPs) could form various building blocks with major capsid proteins (MCPs) before the formation of a viral factory (VF). These results expand our understanding of the capsid assembly of NCVs and provide more targets for vaccine and drug design to fight iridovirid infections. |
リンク | Nat Commun / PubMed:37041173 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.4 - 9.0 Å |
構造データ | EMDB-34227: Cryo-EM structure of Singapore Grouper Iridovirus capsid block 1 EMDB-34229: Cryo-EM structure of Singapore Grouper Iridovirus capsid block 4 EMDB-34230: Cryo-EM structure of Singapore Grouper Iridovirus capsid block 2 EMDB-34235: Cryo-EM structure of Singapore Grouper Iridovirus capsid block 3 EMDB-34236: Cryo-EM structure of Singapore Grouper Iridovirus capsid block 5 EMDB-34251: Singapore Grouper Iridovirus EMDB-34815, PDB-8hif: |
由来 |
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キーワード | VIRAL PROTEIN / Singapore grouper iridovirus / capsid proteins |