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-Structure paper
タイトル | Structure of MotA, a flagellar stator protein, from hyperthermophile. |
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ジャーナル・号・ページ | Biochem Biophys Res Commun, Vol. 631, Page 78-85, Year 2022 |
掲載日 | 2022年11月26日 |
著者 | Tatsuro Nishikino / Norihiro Takekawa / Duy Phuoc Tran / Jun-Ichi Kishikawa / Mika Hirose / Sakura Onoe / Seiji Kojima / Michio Homma / Akio Kitao / Takayuki Kato / Katsumi Imada / |
PubMed 要旨 | Many motile bacteria swim and swarm toward favorable environments using the flagellum, which is rotated by a motor embedded in the inner membrane. The motor is composed of the rotor and the stator, ...Many motile bacteria swim and swarm toward favorable environments using the flagellum, which is rotated by a motor embedded in the inner membrane. The motor is composed of the rotor and the stator, and the motor torque is generated by the change of the interaction between the rotor and the stator induced by the ion flow through the stator. A stator unit consists of two types of membrane proteins termed A and B. Recent cryo-EM studies on the stators from mesophiles revealed that the stator consists of five A and two B subunits, whereas the low-resolution EM analysis showed that purified hyperthermophilic MotA forms a tetramer. To clarify the assembly formation and factors enhancing thermostability of the hyperthermophilic stator, we determined the cryo-EM structure of MotA from Aquifex aeolicus (Aa-MotA), a hyperthermophilic bacterium, at 3.42 Å resolution. Aa-MotA forms a pentamer with pseudo C5 symmetry. A simulated model of the Aa-MotAMotB stator complex resembles the structures of mesophilic stator complexes, suggesting that Aa-MotA can assemble into a pentamer equivalent to the stator complex without MotB. The distribution of hydrophobic residues of MotA pentamers suggests that the extremely hydrophobic nature in the subunit boundary and the transmembrane region is a key factor to stabilize hyperthermophilic Aa-MotA. |
リンク | Biochem Biophys Res Commun / PubMed:36179499 |
手法 | EM (単粒子) |
解像度 | 3.4 Å |
構造データ | EMDB-34203, PDB-8gqy: |
由来 |
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キーワード | MEMBRANE PROTEIN / Bacterial flagellum / stator protein / Aquifex aeolicus / single particle Cryo-EM / MotA |