EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural snapshots uncover a key phosphorylation motif in GPCRs driving β-arrestin activation.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 12, Page 2091-22107.e7, Year 2023
掲載日	2023年6月15日
著者	Jagannath Maharana / Parishmita Sarma / Manish K Yadav / Sayantan Saha / Vinay Singh / Shirsha Saha / Mohamed Chami / Ramanuj Banerjee / Arun K Shukla /
PubMed 要旨	Agonist-induced GPCR phosphorylation is a key determinant for the binding and activation of β-arrestins (βarrs). However, it is not entirely clear how different GPCRs harboring divergent ...Agonist-induced GPCR phosphorylation is a key determinant for the binding and activation of β-arrestins (βarrs). However, it is not entirely clear how different GPCRs harboring divergent phosphorylation patterns impart converging active conformation on βarrs leading to broadly conserved functional responses such as desensitization, endocytosis, and signaling. Here, we present multiple cryo-EM structures of activated βarrs in complex with distinct phosphorylation patterns derived from the carboxyl terminus of different GPCRs. These structures help identify a P-X-P-P type phosphorylation motif in GPCRs that interacts with a spatially organized K-K-R-R-K-K sequence in the N-domain of βarrs. Sequence analysis of the human GPCRome reveals the presence of this phosphorylation pattern in a large number of receptors, and its contribution in βarr activation is demonstrated by targeted mutagenesis experiments combined with an intrabody-based conformational sensor. Taken together, our findings provide important structural insights into the ability of distinct GPCRs to activate βarrs through a significantly conserved mechanism.
リンク	Mol Cell / PubMed:37209686 / PubMed Central
手法	EM (単粒子)
解像度	3.26 - 4.8 Å
構造データ	34173 8go8 EMDB-34173, PDB-8go8: Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1 手法: EM (単粒子) / 解像度: 3.41 Å 34175 8goc EMDB-34175, PDB-8goc: Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human Vasopressin V2 receptor, V2R 手法: EM (単粒子) / 解像度: 4.18 Å 34178 8goo EMDB-34178, PDB-8goo: Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1 手法: EM (単粒子) / 解像度: 4.4 Å 34188 8gp3 EMDB-34188, PDB-8gp3: Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C-X-C chemokine receptor type 4, CXCR4 手法: EM (単粒子) / 解像度: 4.8 Å 35104 8i0n EMDB-35104, PDB-8i0n: Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1 (Local refine) 手法: EM (単粒子) / 解像度: 3.26 Å 35106 8i0q EMDB-35106, PDB-8i0q: Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C-X-C chemokine receptor type 4, CXCR4 (Local refine) 手法: EM (単粒子) / 解像度: 4.45 Å 35114 8i0z EMDB-35114, PDB-8i0z: Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1 (Local refine) 手法: EM (単粒子) / 解像度: 4.33 Å 35115 8i10 EMDB-35115, PDB-8i10: Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human Vasopressin V2 receptor, V2R (Local refine) 手法: EM (単粒子) / 解像度: 3.96 Å
由来	rattus norvegicus (ドブネズミ) homo sapiens (ヒト) mus musculus (ハツカネズミ) bos taurus (ウシ)
キーワード	SIGNALING PROTEIN/IMMUNE SYSTEM / GPCR / Arrestin / SIGNALING PROTEIN / SIGNALING PROTEIN-IMMUNE SYSTEM complex