EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 7, Page 902-913, Year 2023
掲載日	2023年6月1日
著者	Adrien Chauvier / Jason C Porta / Indrajit Deb / Emily Ellinger / Katarina Meze / Aaron T Frank / Melanie D Ohi / Nils G Walter /
PubMed 要旨	Folding of nascent transcripts can be modulated by the RNA polymerase (RNAP) that carries out their transcription, and vice versa. A pause of RNAP during transcription of a preQ riboswitch (termed ...Folding of nascent transcripts can be modulated by the RNA polymerase (RNAP) that carries out their transcription, and vice versa. A pause of RNAP during transcription of a preQ riboswitch (termed que-PEC) is stabilized by a previously characterized template consensus sequence and the ligand-free conformation of the nascent RNA. Ligand binding to the riboswitch induces RNAP pause release and downstream transcription termination; however, the mechanism by which riboswitch folding modulates pausing is unclear. Here, we report single-particle cryo-electron microscopy reconstructions of que-PEC in ligand-free and ligand-bound states. In the absence of preQ, the RNA transcript is in an unexpected hyper-translocated state, preventing downstream nucleotide incorporation. Strikingly, on ligand binding, the riboswitch rotates around its helical axis, expanding the surrounding RNAP exit channel and repositioning the transcript for elongation. Our study reveals the tight coupling by which nascent RNA structures and their ligands can functionally regulate the macromolecular transcription machinery.
リンク	Nat Struct Mol Biol / PubMed:37264140 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 4.3 Å
構造データ	28845 8f3c EMDB-28845, PDB-8f3c: Cryo-EM consensus structure of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand 手法: EM (単粒子) / 解像度: 3.4 Å 29640 8g00 EMDB-29640, PDB-8g00: Cryo-EM structure of 3DVA component 0 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand 手法: EM (単粒子) / 解像度: 3.4 Å 29676 8g1s EMDB-29676, PDB-8g1s: Cryo-EM structure of 3DVA component 1 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand 手法: EM (単粒子) / 解像度: 3.7 Å 29683 8g2w EMDB-29683, PDB-8g2w: Cryo-EM structure of 3DVA component 2 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand 手法: EM (単粒子) / 解像度: 3.7 Å 29732 8g4w EMDB-29732, PDB-8g4w: Cryo-EM consensus structure of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase plus preQ1 ligand 手法: EM (単粒子) / 解像度: 3.8 Å 29812 8g7e EMDB-29812, PDB-8g7e: Cryo-EM structure of 3DVA component 0 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase plus preQ1 ligand 手法: EM (単粒子) / 解像度: 3.9 Å 29859 8g8z EMDB-29859, PDB-8g8z: Cryo-EM structure of 3DVA component 1 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase plus preQ1 ligand 手法: EM (単粒子) / 解像度: 4.3 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PRF: 7-DEAZA-7-AMINOMETHYL-GUANINE / 2-アミノ-5-(アミノメチル)-7H-ピロロ[2,3-d]ピリミジン-4(3H)-オン
由来	escherichia coli (大腸菌) bacillus subtilis (枯草菌)
キーワード	TRANSCRIPTION / RNA Polymerase / Riboswitch / preQ1 / Polymerase / RNAP / aptamer