EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Neck and capsid architecture of the robust Agrobacterium phage Milano.
ジャーナル・号・ページ	Commun Biol, Vol. 6, Issue 1, Page 921, Year 2023
掲載日	2023年9月8日
著者	Ravi R Sonani / Nathaniel C Esteves / Abigail A Horton / Rebecca J Kelly / Amanda L Sebastian / Fengbin Wang / Mark A B Kreutzberger / Petr G Leiman / Birgit E Scharf / Edward H Egelman /
PubMed 要旨	Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is ...Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.
リンク	Commun Biol / PubMed:37684529 / PubMed Central
手法	EM (単粒子)
解像度	2.96 - 4.5 Å
構造データ	29500 8fwb EMDB-29500, PDB-8fwb: Portal assembly of Agrobacterium phage Milano 手法: EM (単粒子) / 解像度: 3.14 Å 29501 8fwc EMDB-29501, PDB-8fwc: Collar sheath structure of Agrobacterium phage Milano 手法: EM (単粒子) / 解像度: 2.96 Å 29503 8fwe EMDB-29503, PDB-8fwe: Neck structure of Agrobacterium phage Milano, C3 symmetry 手法: EM (単粒子) / 解像度: 3.46 Å 29504 8fwg EMDB-29504, PDB-8fwg: Structure of neck and portal vertex of Agrobacterium phage Milano, C5 symmetry 手法: EM (単粒子) / 解像度: 3.45 Å 29512 8fwm EMDB-29512, PDB-8fwm: Structure of tail-neck junction of Agrobacterium phage Milano 手法: EM (単粒子) / 解像度: 3.49 Å 29540 8fxp EMDB-29540, PDB-8fxp: Structure of capsid of Agrobacterium phage Milano 手法: EM (単粒子) / 解像度: 4.04 Å 29541 8fxr EMDB-29541, PDB-8fxr: Structure of neck with portal vertex of capsid of Agrobacterium phage Milano 手法: EM (単粒子) / 解像度: 4.5 Å
由来	agrobacterium phage milano (ファージ)
キーワード	VIRUS / Myophage / redox trigger