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TitleNeck and capsid architecture of the robust Agrobacterium phage Milano.
Journal, issue, pagesCommun Biol, Vol. 6, Issue 1, Page 921, Year 2023
Publish dateSep 8, 2023
AuthorsRavi R Sonani / Nathaniel C Esteves / Abigail A Horton / Rebecca J Kelly / Amanda L Sebastian / Fengbin Wang / Mark A B Kreutzberger / Petr G Leiman / Birgit E Scharf / Edward H Egelman /
PubMed AbstractLarge gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is ...Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.
External linksCommun Biol / PubMed:37684529 / PubMed Central
MethodsEM (single particle)
Resolution2.96 - 4.5 Å
Structure data

29500

8fwb

EMDB-29500, PDB-8fwb:
Portal assembly of Agrobacterium phage Milano
Method: EM (single particle) / Resolution: 3.14 Å

29501

8fwc

EMDB-29501, PDB-8fwc:
Collar sheath structure of Agrobacterium phage Milano
Method: EM (single particle) / Resolution: 2.96 Å

29503

8fwe

EMDB-29503, PDB-8fwe:
Neck structure of Agrobacterium phage Milano, C3 symmetry
Method: EM (single particle) / Resolution: 3.46 Å

29504

8fwg

EMDB-29504, PDB-8fwg:
Structure of neck and portal vertex of Agrobacterium phage Milano, C5 symmetry
Method: EM (single particle) / Resolution: 3.45 Å

29512

8fwm

EMDB-29512, PDB-8fwm:
Structure of tail-neck junction of Agrobacterium phage Milano
Method: EM (single particle) / Resolution: 3.49 Å

29540

8fxp

EMDB-29540, PDB-8fxp:
Structure of capsid of Agrobacterium phage Milano
Method: EM (single particle) / Resolution: 4.04 Å

29541

8fxr

EMDB-29541, PDB-8fxr:
Structure of neck with portal vertex of capsid of Agrobacterium phage Milano
Method: EM (single particle) / Resolution: 4.5 Å

Source
  • agrobacterium phage milano (virus)
KeywordsVIRUS / Myophage / redox trigger

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