+検索条件
-Structure paper
タイトル | A structural dendrogram of the actinobacteriophage major capsid proteins provides important structural insights into the evolution of capsid stability. |
---|---|
ジャーナル・号・ページ | Structure, Vol. 31, Issue 3, Page 282-294.e5, Year 2023 |
掲載日 | 2023年3月2日 |
著者 | Jennifer M Podgorski / Krista Freeman / Sophia Gosselin / Alexis Huet / James F Conway / Mary Bird / John Grecco / Shreya Patel / Deborah Jacobs-Sera / Graham Hatfull / Johann Peter Gogarten / Janne Ravantti / Simon J White / |
PubMed 要旨 | Many double-stranded DNA viruses, including tailed bacteriophages (phages) and herpesviruses, use the HK97-fold in their major capsid protein to make the capsomers of the icosahedral viral capsid. ...Many double-stranded DNA viruses, including tailed bacteriophages (phages) and herpesviruses, use the HK97-fold in their major capsid protein to make the capsomers of the icosahedral viral capsid. After the genome packaging at near-crystalline densities, the capsid is subjected to a major expansion and stabilization step that allows it to withstand environmental stresses and internal high pressure. Several different mechanisms for stabilizing the capsid have been structurally characterized, but how these mechanisms have evolved is still not understood. Using cryo-EM structure determination of 10 capsids, structural comparisons, phylogenetic analyses, and Alphafold predictions, we have constructed a detailed structural dendrogram describing the evolution of capsid structural stability within the actinobacteriophages. We show that the actinobacteriophage major capsid proteins can be classified into 15 groups based upon their HK97-fold. |
リンク | Structure / PubMed:36649709 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.2 - 4.0 Å |
構造データ | EMDB-27824, PDB-8e16: EMDB-27992, PDB-8eb4: EMDB-28012, PDB-8ec2: EMDB-28015, PDB-8ec8: EMDB-28016, PDB-8eci: EMDB-28017, PDB-8ecj: EMDB-28018, PDB-8eck: EMDB-28020, PDB-8ecn: EMDB-28021, PDB-8eco: EMDB-28039, PDB-8edu: |
由来 |
|
キーワード | VIRUS / HK97-fold / T=9 / tailed bacteriophage / T=7 / Bacteriophage / Mycobacteriophage / Capsid |