EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Electron-counting MicroED data with the K2 and K3 direct electron detectors.
ジャーナル・号・ページ	J Struct Biol, Vol. 214, Issue 4, Page 107886, Year 2022
掲載日	2022年8月28日
著者	Max T B Clabbers / Michael W Martynowycz / Johan Hattne / Brent L Nannenga / Tamir Gonen /
PubMed 要旨	Microcrystal electron diffraction (MicroED) uses electron cryo-microscopy (cryo-EM) to collect diffraction data from small crystals during continuous rotation of the sample. As a result of advances ...Microcrystal electron diffraction (MicroED) uses electron cryo-microscopy (cryo-EM) to collect diffraction data from small crystals during continuous rotation of the sample. As a result of advances in hardware as well as methods development, the data quality has continuously improved over the past decade, to the point where even macromolecular structures can be determined ab initio. Detectors suitable for electron diffraction should ideally have fast readout to record data in movie mode, and high sensitivity at low exposure rates to accurately report the intensities. Direct electron detectors are commonly used in cryo-EM imaging for their sensitivity and speed, but despite their availability are generally not used in diffraction. Primary concerns with diffraction experiments are the dynamic range and coincidence loss, which will corrupt the measurement if the flux exceeds the count rate of the detector. Here, we describe instrument setup and low-exposure MicroED data collection in electron-counting mode using K2 and K3 direct electron detectors and show that the integrated intensities can be effectively used to solve structures of two macromolecules between 1.2 Å and 2.8 Å resolution. Even though a beam stop was not used with the K3 studies we did not observe damage to the camera. As these cameras are already available in many cryo-EM facilities, this provides opportunities for users who do not have access to dedicated facilities for MicroED.
リンク	J Struct Biol / PubMed:36044956 / PubMed Central
手法	EM (電子線結晶学)
解像度	1.2 - 2.8 Å
構造データ	27900 8e52 EMDB-27900, PDB-8e52: MicroED structure of proteinase K recorded on K2 手法: EM (電子線結晶学) 27901 8e53 EMDB-27901, PDB-8e53: MicroED structure of proteinase K recorded on K3 手法: EM (電子線結晶学) 27902 8e54 EMDB-27902, PDB-8e54: MicroED structure of triclinic lysozyme recorded on K3 手法: EM (電子線結晶学)
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-HOH: WATER ChemComp-NO3: NITRATE ION
由来	parengyodontium album (菌類) gallus gallus (ニワトリ) chicken (ニワトリ)
キーワード	HYDROLASE / serine protease