EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.
ジャーナル・号・ページ	Mol Cell, Vol. 82, Issue 21, Page 4049-44063.e6, Year 2022
掲載日	2022年11月3日
著者	Karina Jouravleva / Dmitrij Golovenko / Gabriel Demo / Robert C Dutcher / Traci M Tanaka Hall / Phillip D Zamore / Andrei A Korostelev /
PubMed 要旨	In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM ...In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs‑PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer‑1⋅Loqs‑PB heterodimer. The Dicer-1 dsRBD and three Loqs‑PB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicer‑1⋅Loqs‑PB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release.
リンク	Mol Cell / PubMed:36182693 / PubMed Central
手法	EM (単粒子)
解像度	3.06 - 4.02 Å
構造データ	27415 8dfv EMDB-27415, PDB-8dfv: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIa 手法: EM (単粒子) / 解像度: 3.06 Å 27416 8dg5 EMDB-27416, PDB-8dg5: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIb 手法: EM (単粒子) / 解像度: 3.26 Å 27417 8dg7 EMDB-27417, PDB-8dg7: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex III 手法: EM (単粒子) / 解像度: 3.32 Å 27420 8dga EMDB-27420, PDB-8dga: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IV 手法: EM (単粒子) / 解像度: 3.73 Å 27423 8dgi EMDB-27423, PDB-8dgi: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex Ia 手法: EM (単粒子) / 解像度: 3.94 Å 27427 8dgj EMDB-27427, PDB-8dgj: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex Ib 手法: EM (単粒子) / 解像度: 4.02 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-U5P: URIDINE-5'-MONOPHOSPHATE / UMP / ウリジル酸
由来	drosophila melanogaster (キイロショウジョウバエ)
キーワード	RNA BINDING PROTEIN/RNA / Dicer (Dicer) / Dcr-1 (Dicer) / Loquacious / Loqs-P / miRNA / RNA BINDING PROTEIN-RNA complex / Loqs-PB