EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the assembly of gp130 family cytokine signaling complexes.
ジャーナル・号・ページ	Sci Adv, Vol. 9, Issue 11, Page eade4395, Year 2023
掲載日	2023年3月15日
著者	Yi Zhou / Panayiotis E Stevis / Jing Cao / Kei Saotome / Jiaxi Wu / Arielle Glatman Zaretsky / Sokol Haxhinasto / George D Yancopoulos / Andrew J Murphy / Mark W Sleeman / William C Olson / Matthew C Franklin /
PubMed 要旨	The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling receptor and play crucial roles in various cellular processes. ...The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling receptor and play crucial roles in various cellular processes. We determined cryo-electron microscopy structures of five signaling complexes of this family, containing full receptor ectodomains bound to their respective ligands ciliary neurotrophic factor, cardiotrophin-like cytokine factor 1 (CLCF1), leukemia inhibitory factor, IL-27, and IL-6. Our structures collectively reveal similarities and differences in the assembly of these complexes. The acute bends at both signaling receptors in all complexes bring the membrane-proximal domains to a ~30 angstrom range but with distinct distances and orientations. We also reveal how CLCF1 engages its secretion chaperone cytokine receptor-like factor 1. Our data provide valuable insights for therapeutically targeting gp130-mediated signaling.
リンク	Sci Adv / PubMed:36930708 / PubMed Central
手法	EM (単粒子)
解像度	2.93 - 4.14 Å
構造データ	27221 8d6a EMDB-27221: Cryo-EM map of human LIF signaling complex with full extracellular domains PDB-8d6a: Cryo-EM structure of human LIF signaling complex: model containing the interaction core region 手法: EM (単粒子) / 解像度: 3.54 Å 27227 8d74 EMDB-27227: Cryo-EM map of human CNTF signaling complex with full extracellular domains: refined from selected full particle dataset, with better resolution in the interaction core region PDB-8d74: Cryo-EM structure of human CNTF signaling complex: model containing the interaction core region 手法: EM (単粒子) / 解像度: 3.03 Å 27228 8d7e EMDB-27228, PDB-8d7e: Cryo-EM structure of human CNTFR alpha in complex with the Fab fragments of two antibodies 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-27229: Cryo-EM map of human CNTF signaling complex with full extracellular domains: refined from a particle subset, with lower global resolution but improved LIFR D6-D8 density 手法: EM (単粒子) / 解像度: 3.37 Å 27230 8d7h EMDB-27230, PDB-8d7h: Cryo-EM structure of human CLCF1 in complex with CRLF1 and CNTFR alpha 手法: EM (単粒子) / 解像度: 3.4 Å 27231 8d7r EMDB-27231: Cryo-EM map of human CLCF1 signaling complex with full extracellular domains PDB-8d7r: Cryo-EM structure of human CLCF1 signaling complex: model containing the interaction core region 手法: EM (単粒子) / 解像度: 3.9 Å 27244 8d82 EMDB-27244: Cryo-EM map of detergent-solubilized human IL-6 signaling complex with full extracellular domains PDB-8d82: Cryo-EM structure of human IL-6 signaling complex in detergent: model containing full extracellular domains 手法: EM (単粒子) / 解像度: 3.22 Å EMDB-27246: Cryo-EM map of human IL-27 signaling complex with full extracellular domains 手法: EM (単粒子) / 解像度: 4.14 Å 27247 8d85 EMDB-27247: Cryo-EM map of human IL-27 signaling complex: focused refinement on the interaction core region PDB-8d85: Cryo-EM structure of human IL-27 signaling complex: model containing the interaction core region 手法: EM (単粒子) / 解像度: 3.81 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	homo sapiens (ヒト)
キーワード	CYTOKINE / cytokine signaling / LIF / gp130 / LIFR / CNTF / CNTFR alpha / CYTOKINE/Immune System / antibody / Fab / CYTOKINE-Immune System complex / CLCF1 / CRLF1 / IL-6 / IL-6R alpha / IL-27 / IL-27R alpha / EBI3 / p28