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-Structure paper
タイトル | The bacterial replication origin BUS promotes nucleobase capture. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 8339, Year 2023 |
掲載日 | 2023年12月14日 |
![]() | Simone Pelliciari / Salomé Bodet-Lefèvre / Stepan Fenyk / Daniel Stevens / Charles Winterhalter / Frederic D Schramm / Sara Pintar / Daniel R Burnham / George Merces / Tomas T Richardson / Yumiko Tashiro / Julia Hubbard / Hasan Yardimci / Aravindan Ilangovan / Heath Murray / ![]() |
PubMed 要旨 | Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is ...Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (oriC) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that Bacillus subtilis DnaA assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS). |
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手法 | EM (単粒子) / X線回折 |
解像度 | 2.38 - 3.2 Å |
構造データ | EMDB-16229, PDB-8btg: ![]() PDB-8bv3: |
化合物 | ![]() ChemComp-MG: ![]() ChemComp-ATP: ![]() ChemComp-ANP: ![]() ChemComp-K: ![]() ChemComp-HOH: |
由来 |
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![]() | REPLICATION / DnaA / cryo-EM / BUS complex / replication initiation / CELL CYCLE / DNA replication / DNA replication initiation / AAA+ superfamily / Initiator Clade |