EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Unraveling the maturation pathway of a eukaryotic virus through cryo-EM.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 123, Issue 9, Page e2420493123, Year 2026
掲載日	2026年3月3日
著者	Roger Castells-Graells / Emma L Hesketh / Tsutomu Matsui / John E Johnson / Neil A Ranson / David M Lawson / George P Lomonossoff /
PubMed 要旨	Virus maturation is a fundamental biological process involving large-scale structural reorganizations that drive functional activation and lead to infectivity. Understanding the steps from the ...Virus maturation is a fundamental biological process involving large-scale structural reorganizations that drive functional activation and lead to infectivity. Understanding the steps from the initial procapsid assembly to mature virions is essential, both for comprehending viral life cycles and for developing antiviral therapies. However, capturing these steps has been challenging due to the transient and elusive nature of intermediate states. The nonenveloped, T = 4, ssRNA-containing, omega virus (NωV) is a highly accessible model system that exemplifies the maturation process of a eukaryotic virus. During maturation, the particle shrinks in outer diameter from 482 Å (pH 7.6) to 428 Å (pH 5.0). It is possible to mimic the maturation process in vitro by lowering the pH of a population of procapsids produced in heterologous systems. Indeed, by controlling the pH in vitro, it is possible to produce homogenous populations of intermediate NωV virus-like particles (VLPs) that occur too fleetingly to be observed in vivo. Here, we report structural models, based on cryoelectron microscopy (cryo-EM), of five intermediates in the NωV maturation process. The structures of the intermediate particles reveal unique, quaternary position-dependent trajectories and refolding of subunit N and C-terminal regions, including the formation of the autocatalytic cleavage site at N570. The detailed structures reported here, coupled with previously determined structures of the procapsids and mature particles, allow the maturation pathway to be described in detail for a eukaryotic virus.
リンク	Proc Natl Acad Sci U S A / PubMed:41739563 / PubMed Central
手法	EM (単粒子)
解像度	3.39 - 4.88 Å
構造データ	15112 8a3c EMDB-15112, PDB-8a3c: Nudaurelia capensis omega virus maturation intermediate captured at pH5.9 (insect cell expressed VLPs) 手法: EM (単粒子) / 解像度: 3.92 Å 15134 8a41 EMDB-15134, PDB-8a41: Nudaurelia capensis omega virus procapsid at pH7.6 (insect cell expressed VLPs) 手法: EM (単粒子) / 解像度: 4.88 Å 15209 8a6j EMDB-15209, PDB-8a6j: Nudaurelia capensis omega virus maturation intermediate captured at pH6.25 (insect cell expressed VLPs) 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-15266: Nudaurelia capensis omega virus maturation intermediates captured at pH5.6 (insect cell expressed VLPs): consensus map 手法: EM (単粒子) / 解像度: 3.39 Å 15307 8aay EMDB-15307, PDB-8aay: Nudaurelia capensis omega virus maturation intermediate captured at pH5.6 (insect cell expressed VLPs): small class from symmetry expansion 手法: EM (単粒子) / 解像度: 3.39 Å 15339 8ac6 EMDB-15339, PDB-8ac6: Nudaurelia capensis omega virus maturation intermediate captured at pH5.6 (insect cell expressed VLPs): medium class from symmetry expansion 手法: EM (単粒子) / 解像度: 3.63 Å 15348 8ach EMDB-15348, PDB-8ach: Nudaurelia capensis omega virus maturation intermediate captured at pH5.6 (insect cell expressed VLPs): large class from symmetry expansion 手法: EM (単粒子) / 解像度: 3.91 Å
由来	nudaurelia capensis omega virus (ウイルス)
キーワード	VIRUS LIKE PARTICLE / ICOSAHEDRAL VIRUS / AUTO-CATALYTIC CLEAVAGE / VIRUS MATURATION / VIRUS-LIKE PARTICLE