EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 14, Page 2493-22508.e5, Year 2023
掲載日	2023年7月20日
著者	Ning Cui / Jun-Tao Zhang / Yongrui Liu / Yanhong Liu / Xiao-Yu Liu / Chongyuan Wang / Hongda Huang / Ning Jia /
PubMed 要旨	Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types for which the mechanistic ...Type IV CRISPR-Cas systems, which are primarily found on plasmids and exhibit a strong plasmid-targeting preference, are the only one of the six known CRISPR-Cas types for which the mechanistic details of their function remain unknown. Here, we provide high-resolution functional snapshots of type IV-A Csf complexes before and after target dsDNA binding, either in the absence or presence of CasDinG, revealing the mechanisms underlying Csf complex assembly, "DWN" PAM-dependent dsDNA targeting, R-loop formation, and CasDinG recruitment. Furthermore, we establish that CasDinG, a signature DinG family helicase, harbors ssDNA-stimulated ATPase activity and ATP-dependent 5'-3' DNA helicase activity. In addition, we show that CasDinG unwinds the non-target strand (NTS) and target strand (TS) of target dsDNA from the Csf complex. These molecular details advance our mechanistic understanding of type IV-A CRISPR-Csf function and should enable Csf complexes to be harnessed as genome-engineering tools for biotechnological applications.
リンク	Mol Cell / PubMed:37343553
手法	EM (単粒子) / X線回折
解像度	2.6 - 3.7 Å
構造データ	33180 7xfz EMDB-33180, PDB-7xfz: CryoEM structure of type IV-A Csf-crRNAsp14-dsDNA ternary complex 手法: EM (単粒子) / 解像度: 3.0 Å 33181 7xg0 EMDB-33181, PDB-7xg0: CryoEM structure of type IV-A Csf-crRNA-dsDNA ternary complex 手法: EM (単粒子) / 解像度: 2.6 Å 33183 7xg2 EMDB-33183, PDB-7xg2: CryoEM structure of type IV-A NTS-nicked dsDNA bound Csf-crRNA ternary complex 手法: EM (単粒子) / 解像度: 2.8 Å 33184 7xg3 EMDB-33184, PDB-7xg3: CryoEM structure of type IV-A CasDinG bound NTS-nicked Csf-crRNA-dsDNA quaternary complex 手法: EM (単粒子) / 解像度: 3.0 Å 33185 7xg4 EMDB-33185, PDB-7xg4: CryoEM structure of type IV-A CasDinG bound NTS-nicked Csf-crRNA-dsDNA quaternary complex in a second state 手法: EM (単粒子) / 解像度: 3.7 Å PDB-7xex: Crystal strucutre of apoCasDinG 手法: X-RAY DIFFRACTION / 解像度: 3 Å PDB-7xf0: Crystal strucutre of CasDinG in complex with ATP 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å PDB-7xf1: Crystal strucutre of apoCasDinG in complex with ssDNA 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ZN*: Unknown entry
由来	pseudomonas aeruginosa (緑膿菌) synthetic construct (人工物)
キーワード	STRUCTURAL PROTEIN / Nuclease / STRUCTURAL PROTEIN/DNA / STRUCTURAL PROTEIN-DNA complex / STRUCTURAL PROTEIN/RNA/DNA / STRUCTURAL PROTEIN-RNA-DNA COMPLEX