EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution structures of human Na1.7 reveal gating modulation through α-π helical transition of S6.
ジャーナル・号・ページ	Cell Rep, Vol. 39, Issue 4, Page 110735, Year 2022
掲載日	2022年4月26日
著者	Gaoxingyu Huang / Dongliang Liu / Weipeng Wang / Qiurong Wu / Jiaofeng Chen / Xiaojing Pan / Huaizong Shen / Nieng Yan /
PubMed 要旨	Na1.7 represents a preeminent target for next-generation analgesics for its critical role in pain sensation. Here we report a 2.2-Å resolution cryo-EM structure of wild-type (WT) Na1.7 complexed ...Na1.7 represents a preeminent target for next-generation analgesics for its critical role in pain sensation. Here we report a 2.2-Å resolution cryo-EM structure of wild-type (WT) Na1.7 complexed with the β1 and β2 subunits that reveals several previously indiscernible cytosolic segments. Reprocessing of the cryo-EM data for our reported structures of Na1.7(E406K) bound to various toxins identifies two distinct conformations of S6, one composed of α helical turns only and the other containing a π helical turn in the middle. The structure of ligand-free Na1.7(E406K), determined at 3.5-Å resolution, is identical to the WT channel, confirming that binding of Huwentoxin IV or Protoxin II to VSD allosterically induces the α → π transition of S6. The local secondary structural shift leads to contraction of the intracellular gate, closure of the fenestration on the interface of repeats I and IV, and rearrangement of the binding site for the fast inactivation motif.
リンク	Cell Rep / PubMed:35476982
手法	EM (単粒子)
解像度	2.2 - 3.5 Å
構造データ	32368 7w9k EMDB-32368, PDB-7w9k: Cryo-EM structure of human Nav1.7-beta1-beta2 complex at 2.2 angstrom resolution 手法: EM (単粒子) / 解像度: 2.2 Å 32369 7w9l EMDB-32369, PDB-7w9l: Cryo-EM structure of human Nav1.7(E406K)-beta1-beta2 complex 手法: EM (単粒子) / 解像度: 3.5 Å 32370 7w9m EMDB-32370, PDB-7w9m: Cryo-EM structure of human Nav1.7(E406K) in complex with auxiliary beta subunits, ProTx-II and tetrodotoxin (S6IV pi helix conformer) 手法: EM (単粒子) / 解像度: 3.0 Å 32371 7w9p EMDB-32371, PDB-7w9p: Cryo-EM structure of human Nav1.7(E406K) in complex with auxiliary beta subunits, huwentoxin-IV and saxitoxin (S6IV pi helix conformer) 手法: EM (単粒子) / 解像度: 2.9 Å 32372 7w9t EMDB-32372, PDB-7w9t: Cryo-EM structure of human Nav1.7(E406K) in complex with auxiliary beta subunits, huwentoxin-IV and saxitoxin (S6IV alpha helix conformer) 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / O-(1-O,2-O-ジステアロイル-L-グリセロ-3-ホスホ)セリン / ホスファチジルセリン ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト ChemComp-9Z9: (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en / 可溶化剤YM ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-LPE: 1-O-OCTADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE / O-(1-O-オクタデシル-L-グリセロ-3-ホスホ)コリン ChemComp-1PW: (2S,3R,4E)-2-(acetylamino)-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate / C₂-セラミド1-ホスファ-ト ChemComp-PCW: 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / O-(1-O,2-O-ジオレオイル-L-グリセロ-3-ホスホ)コリン / DOPC, リン脂質YM ChemComp-HOH: WATER / 水 ChemComp-9SR: (1R,5R,6R,7R,9S,11S,12S,13S,14S)-3-amino-14-(hydroxymethyl)-8,10-dioxa-2,4-diazatetracyclo[7.3.1.1~7,11~.0~1,6~]tetradec-3-ene-5,9,12,13,14-pentol (non-preferred name) / テトロドトキシン / 神経毒YM / テトロドトキシン ChemComp-9SL: [(3aS,4R,10aS)-2,6-diamino-10,10-dihydroxy-3a,4,9,10-tetrahydro-3H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate / サキシトキシン / 神経毒YM / サキシトキシン
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Nav1.7 / SCN9A / cryo-EM (低温電子顕微鏡法) / voltage gated sodium channel (ナトリウムチャネル)