EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures reveal that RFC recognizes both the 3'- and 5'-DNA ends to load PCNA onto gaps for DNA repair.
ジャーナル・号・ページ	Elife, Vol. 11, Year 2022
掲載日	2022年7月13日
著者	Fengwei Zheng / Roxana Georgescu / Nina Y Yao / Huilin Li / Michael E O'Donnell /
PubMed 要旨	RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3' ss/ds DNA junctions to load PCNA onto DNA during ...RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3' ss/ds DNA junctions to load PCNA onto DNA during replication. We show here five structures that identify a second DNA binding site in RFC that binds a 5' duplex. This 5' DNA site is located between the N-terminal BRCT domain and AAA+ module of the large Rfc1 subunit. Our structures reveal ideal binding to a 7-nt gap, which includes 2 bp unwound by the clamp loader. Biochemical studies show enhanced binding to 5 and 10 nt gaps, consistent with the structural results. Because both 3' and 5' ends are present at a ssDNA gap, we propose that the 5' site facilitates RFC's PCNA loading activity at a DNA damage-induced gap to recruit gap-filling polymerases. These findings are consistent with genetic studies showing that base excision repair of gaps greater than 1 base requires PCNA and involves the 5' DNA binding domain of Rfc1. We further observe that a 5' end facilitates PCNA loading at an RPA coated 30-nt gap, suggesting a potential role of the RFC 5'-DNA site in lagging strand DNA synthesis.
リンク	Elife / PubMed:35829698 / PubMed Central
手法	EM (単粒子)
解像度	3.09 - 3.41 Å
構造データ	25872 7tfh EMDB-25872: Cryo-EM 3D map of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to two DNA molecules, one at the 5'-recessed end and the other at the 3'-recessed end PDB-7tfh: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to two DNA molecules, one at the 5'-recessed end and the other at the 3'-recessed end 手法: EM (単粒子) / 解像度: 3.09 Å 25873 7tfi EMDB-25873: Cryo-EM 3D map of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp PDB-7tfi: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp 手法: EM (単粒子) / 解像度: 3.41 Å 25874 7tfj EMDB-25874: Cryo-EM 3D map of S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with a closed clamp ring PDB-7tfj: Atomic model of S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with a closed clamp ring 手法: EM (単粒子) / 解像度: 3.3 Å 25875 7tfk EMDB-25875: Cryo-EM 3D map of S. cerevisiae clamp loader RFC bound to two DNA molecules, one at the 5'-recessed end and the other at the 3'-recessed end PDB-7tfk: Atomic model of S. cerevisiae clamp loader RFC bound to two DNA molecules, one at the 5'-recessed end and the other at the 3'-recessed end 手法: EM (単粒子) / 解像度: 3.25 Å 25876 7tfl EMDB-25876: Cryo-EM 3D map of S. cerevisiae clamp loader RFC bound to DNA PDB-7tfl: Atomic model of S. cerevisiae clamp loader RFC bound to DNA 手法: EM (単粒子) / 解像度: 3.33 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	synthetic construct (人工物) saccharomyces cerevisiae (パン酵母)
キーワード	DNA BINDING PROTEIN/DNA / DNA replication / DNA damage repair / RFC loader / PCNA clamp / DNA polymerase processivity factor / DNA BINDING PROTEIN-DNA complex