EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture and self-assembly of the jumbo bacteriophage nuclear shell.
ジャーナル・号・ページ	Nature, Vol. 608, Issue 7922, Page 429-435, Year 2022
掲載日	2022年8月3日
著者	Thomas G Laughlin / Amar Deep / Amy M Prichard / Christian Seitz / Yajie Gu / Eray Enustun / Sergey Suslov / Kanika Khanna / Erica A Birkholz / Emily Armbruster / J Andrew McCammon / Rommie E Amaro / Joe Pogliano / Kevin D Corbett / Elizabeth Villa /
PubMed 要旨	Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a ...Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors. However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
リンク	Nature / PubMed:35922510 / PubMed Central
手法	EM (サブトモグラム平均) / EM (単粒子)
解像度	2.3 - 27.0 Å
構造データ	EMDB-25183: P. chlororaphis 70S ribosome in situ subtomogram average 手法: EM (サブトモグラム平均) / 解像度: 10.2 Å EMDB-25220: In situ subtomogram average of the 201phi2-1 phage nucleus major shell protein, chimallin (concave class) 手法: EM (サブトモグラム平均) / 解像度: 20.0 Å EMDB-25221: In situ consensus subtomogram average of the 201phi2-1 chimallin 手法: EM (サブトモグラム平均) / 解像度: 24.0 Å EMDB-25222: In situ subtomogram average of 201phi2-1 phage nucleus major shell protein, chimallin (intermediate/flat class) 手法: EM (サブトモグラム平均) / 解像度: 18.0 Å EMDB-25223: In situ subtomogram average of the 201phi2-1 phage nucleus major shell protein, chimallin (convex class) 手法: EM (サブトモグラム平均) / 解像度: 23.0 Å EMDB-25229: In situ subtomogram average of the Goslar major phage nucleus shell protein, chimallin (consensus class) 手法: EM (サブトモグラム平均) / 解像度: 27.0 Å EMDB-25262: In situ subtomogram average of Goslar phage nucleus major shell protein, chimallin (concave class) 手法: EM (サブトモグラム平均) / 解像度: 20.0 Å EMDB-25358: In situ subtomogram average of the major Goslar phage nucleus shell protein, chimallin (convex class) 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-25359: In situ subtomogram average of the APEC2248 70S ribosome 手法: EM (サブトモグラム平均) / 解像度: 8.53 Å EMDB-25360: In situ subtomogram average of the APEC2248 50S ribosome 手法: EM (サブトモグラム平均) / 解像度: 12.0 Å 25390 7sqq EMDB-25390, PDB-7sqq: 201Phi2-1 Chimallin Cubic (O, 24mer) assembly 手法: EM (単粒子) / 解像度: 4.2 Å 25391 7sqr EMDB-25391, PDB-7sqr: 201phi2-1 Chimallin localized tetramer reconstruction 手法: EM (単粒子) / 解像度: 3.4 Å 25392 7sqs EMDB-25392, PDB-7sqs: 201phi2-1 Chimallin C1 localized reconstruction 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-25393: 201phi2-1 chimallin rectangular (D4,40mer) assembly 手法: EM (単粒子) / 解像度: 21.0 Å 25394 7sqt EMDB-25394, PDB-7sqt: Goslar chimallin cubic (O, 24mer) assembly 手法: EM (単粒子) / 解像度: 4.0 Å 25395 7squ EMDB-25395, PDB-7squ: Goslar chimallin C4 tetramer localized reconstruction 手法: EM (単粒子) / 解像度: 2.6 Å 25396 7sqv EMDB-25396, PDB-7sqv: Goslar chimallin C1 localized reconstruction 手法: EM (単粒子) / 解像度: 2.3 Å
化合物	ChemComp-HOH: WATER
由来	Pseudomonas chlororaphis (バクテリア) pseudomonas phage 201phi2-1 (ファージ) Goslarvirus (ウイルス) Escherichia coli (大腸菌) escherichia phage vb_ecom_goslar (ファージ)
キーワード	STRUCTURAL PROTEIN / Phage / virus / viral protein