EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure and rRNA modification sites of a plant ribosome.
ジャーナル・号・ページ	Plant Commun, Vol. 3, Issue 5, Page 100342, Year 2022
掲載日	2022年9月12日
著者	Patrick Cottilli / Yuzuru Itoh / Yuko Nobe / Anton S Petrov / Purificación Lisón / Masato Taoka / Alexey Amunts /
PubMed 要旨	Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to ...Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to changing environmental cues via the deposition of chemical modifications into rRNA. Here, we present the structure of a plant ribosome from tomato and a quantitative mass spectrometry analysis of its rRNAs. The study reveals fine features of the ribosomal proteins and 71 plant-specific rRNA modifications, and it re-annotates 30 rRNA residues in the available sequence. At the protein level, isoAsp is found in position 137 of uS11, and a zinc finger previously believed to be universal is missing from eL34, suggesting a lower effect of zinc deficiency on protein synthesis in plants. At the rRNA level, the plant ribosome differs markedly from its human counterpart with respect to the spatial distribution of modifications. Thus, it represents an additional layer of gene expression regulation, highlighting the molecular signature of a plant ribosome. The results provide a reference model of a plant ribosome for structural studies and an accurate marker for molecular ecology.
リンク	Plant Commun / PubMed:35643637 / PubMed Central
手法	EM (単粒子)
解像度	2.35 - 2.8 Å
構造データ	14001 7qiw EMDB-14001, PDB-7qiw: Specific features and methylation sites of a plant ribosome. 60S ribosomal subunit. 手法: EM (単粒子) / 解像度: 2.35 Å 14002 7qix EMDB-14002, PDB-7qix: Specific features and methylation sites of a plant ribosome. 40S body ribosomal subunit. 手法: EM (単粒子) / 解像度: 2.53 Å 14003 7qiy EMDB-14003: Specific features and methylation sites of a plant ribosome PDB-7qiy: Specific features and methylation sites of a plant ribosome. 40S head ribosomal subunit. 手法: EM (単粒子) / 解像度: 2.58 Å 14004 7qiz EMDB-14004, PDB-7qiz: Specific features and methylation sites of a plant 80S ribosome 手法: EM (単粒子) / 解像度: 2.38 Å EMDB-14051: Specific features and methylation sites of a plant ribosome. 80S translating ribosome (A/A-P/P-E/E). 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-14052: Specific features and methylation sites of a plant ribosome. 80S translating ribosome (A/P-P/E). 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-32404: Specific features and methylation sites of a plant ribosome. 80S ribosome consensus map. 手法: EM (単粒子) / 解像度: 2.38 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-SPM: SPERMINE / スペルミン ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-HOH: WATER ChemComp-PUT: 1,4-DIAMINOBUTANE / プトレシン ChemComp-BGC: beta-D-glucopyranose / β-D-グルコピラノ-ス
由来	solanum lycopersicum (トマト)
キーワード	RIBOSOME / Solanum lycopersicum / cytosolic ribosome / 80S / plant / rRNA