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-Structure paper
タイトル | Mitoribosomal small subunit maturation involves formation of initiation-like complexes. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 119, Issue 3, Year 2022 |
掲載日 | 2022年1月18日 |
著者 | Tea Lenarčič / Moritz Niemann / David J F Ramrath / Salvatore Calderaro / Timo Flügel / Martin Saurer / Marc Leibundgut / Daniel Boehringer / Céline Prange / Elke K Horn / André Schneider / Nenad Ban / |
PubMed 要旨 | Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different ...Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that mitoribosome maturation follows common principles and involves a number of conserved assembly factors. To investigate the steps involved in the assembly of the mitoribosomal small subunit (mt-SSU) we determined the cryoelectron microscopy structures of middle and late assembly intermediates of the mitochondrial small subunit (mt-SSU) at 3.6- and 3.7-Å resolution, respectively. We identified five additional assembly factors that together with the mitochondrial initiation factor 2 (mt-IF-2) specifically interact with functionally important regions of the rRNA, including the decoding center, thereby preventing premature mRNA or large subunit binding. Structural comparison of assembly intermediates with mature mt-SSU combined with RNAi experiments suggests a noncanonical role of mt-IF-2 and a stepwise assembly process, where modular exchange of ribosomal proteins and assembly factors together with mt-IF-2 ensure proper 9S rRNA folding and protein maturation during the final steps of assembly. |
リンク | Proc Natl Acad Sci U S A / PubMed:35042777 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.6 - 3.7 Å |
構造データ | EMDB-13660, PDB-7pua: EMDB-13661, PDB-7pub: |
化合物 | ChemComp-MG: ChemComp-ATP: ChemComp-PO4: ChemComp-PM8: ChemComp-ZN: ChemComp-FDA: ChemComp-GDP: ChemComp-HOH: ChemComp-UTP: ChemComp-FAD: |
由来 |
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キーワード | RIBOSOME / mitoribosome / assembly intermediate / Trypanosoma brucei / small subunit / mt-SSU / SSU |