EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
ジャーナル・号・ページ	PLoS One, Vol. 16, Issue 11, Page e0252497, Year 2021
掲載日	2021年11月23日
著者	Gisela Pöll / Michael Pilsl / Joachim Griesenbeck / Herbert Tschochner / Philipp Milkereit /
PubMed 要旨	In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with ...In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with the stabilisation and maturation of subunit precursors potentially promotes the production of ribosomes with defined composition. To further decipher mechanisms of such an intrinsic quality control pathway we analysed here the contribution of three yeast large ribosomal subunit r-proteins rpL2 (uL2), rpL25 (uL23) and rpL34 (eL34) for intermediate nuclear subunit folding steps. Structure models obtained from single particle cryo-electron microscopy analyses provided evidence for specific and hierarchic effects on the stable positioning and remodelling of large ribosomal subunit domains. Based on these structural and previous biochemical data we discuss possible mechanisms of r-protein dependent hierarchic domain arrangement and the resulting impact on the stability of misassembled subunits.
リンク	PLoS One / PubMed:34813592 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.72 Å
構造データ	12866 7of1 EMDB-12866, PDB-7of1: Nog1-TAP associated immature ribosomal particle population A from S. cerevisiae 手法: EM (単粒子) / 解像度: 3.1 Å 12892 7oh3 EMDB-12892, PDB-7oh3: Nog1-TAP associated immature ribosomal particle population B from S. cerevisiae 手法: EM (単粒子) / 解像度: 3.4 Å 12904 7ohp EMDB-12904, PDB-7ohp: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL25 expression shut down, population A 手法: EM (単粒子) / 解像度: 3.9 Å 12905 7ohq EMDB-12905, PDB-7ohq: Nog1-TAP associated immature ribosomal particle population C from S. cerevisiae 手法: EM (単粒子) / 解像度: 3.1 Å 12906 7ohr EMDB-12906, PDB-7ohr: Nog1-TAP associated immature ribosomal particle population E from S. cerevisiae 手法: EM (単粒子) / 解像度: 4.72 Å 12907 7ohs EMDB-12907, PDB-7ohs: Nog1-TAP associated immature ribosomal particle population F from S. cerevisiae 手法: EM (単粒子) / 解像度: 4.38 Å 12908 7oht EMDB-12908, PDB-7oht: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population A 手法: EM (単粒子) / 解像度: 4.7 Å 12909 7ohu EMDB-12909, PDB-7ohu: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population B 手法: EM (単粒子) / 解像度: 3.7 Å 12910 7ohv EMDB-12910, PDB-7ohv: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population C 手法: EM (単粒子) / 解像度: 3.9 Å 12911 7ohw EMDB-12911, PDB-7ohw: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL25 expression shut down, population B 手法: EM (単粒子) / 解像度: 3.5 Å 12912 7ohx EMDB-12912, PDB-7ohx: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A 手法: EM (単粒子) / 解像度: 3.3 Å 12913 7ohy EMDB-12913, PDB-7ohy: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population B 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	RIBOSOME / ribosomal assembly state