EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Pore dynamics and asymmetric cargo loading in an encapsulin nanocompartment.
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 4, Page eabj4461, Year 2022
掲載日	2022年1月28日
著者	Jennifer Ross / Zak McIver / Thomas Lambert / Cecilia Piergentili / Jasmine Emma Bird / Kelly J Gallagher / Faye L Cruickshank / Patrick James / Efrain Zarazúa-Arvizu / Louise E Horsfall / Kevin J Waldron / Marcus D Wilson / C Logan Mackay / Arnaud Baslé / David J Clarke / Jon Marles-Wright /
PubMed 要旨	Encapsulins are protein nanocompartments that house various cargo enzymes, including a family of decameric ferritin-like proteins. Here, we study a recombinant encapsulin:encapsulated ferritin ...Encapsulins are protein nanocompartments that house various cargo enzymes, including a family of decameric ferritin-like proteins. Here, we study a recombinant encapsulin:encapsulated ferritin complex using cryo-electron microscopy and hydrogen/deuterium exchange mass spectrometry to gain insight into the structural relationship between the encapsulin shell and its protein cargo. An asymmetric single-particle reconstruction reveals four encapsulated ferritin decamers in a tetrahedral arrangement within the encapsulin nanocompartment. This leads to a symmetry mismatch between the protein cargo and the icosahedral encapsulin shell. The encapsulated ferritin decamers are offset from the interior face of the encapsulin shell. Using hydrogen/deuterium exchange mass spectrometry, we observed the dynamic behavior of the major fivefold pore in the encapsulin shell and show the pore opening via the movement of the encapsulin A-domain. These data will accelerate efforts to engineer the encapsulation of heterologous cargo proteins and to alter the permeability of the encapsulin shell via pore modifications.
リンク	Sci Adv / PubMed:35080974 / PubMed Central
手法	EM (単粒子)
解像度	2.4 - 5.5 Å
構造データ	12853 7odw EMDB-12853: Icosahedral cryo-EM reconstruction of Haliangium ochraceum encapsulin PDB-7odw: Model of Haliangium ochraceum encapsulin from icosahedral single particle reconstruction 手法: EM (単粒子) / 解像度: 2.5 Å 12859 7oe2 EMDB-12859, PDB-7oe2: Model of closed pentamer of the Haliangium ochraceum encapsulin from symmetry expansion of icosahedral single particle reconstruction 手法: EM (単粒子) / 解像度: 2.4 Å 12864 7oeu EMDB-12864: Single particle reconstruction of open pentamer of the Haliangium ochraceum encapsulin from symmetry expansion of icosahedral single particle reconstruction PDB-7oeu: Model of open pentamer of the Haliangium ochraceum encapsulin from symmetry expansion of icosahedral single particle reconstruction 手法: EM (単粒子) / 解像度: 2.64 Å EMDB-12873: Asymmetric single particle reconstruction of the Haliangium ochraceum encapsulin encapsulated ferritin complex 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-13603: Asymmetric single particle reconstruction of the Haliangium ochraceum encapsulin encapsulated ferritin complex calculated using Cryosparc 手法: EM (単粒子) / 解像度: 3.24 Å EMDB-13608: Focused reconstruction of Haliangium ochraceum encapsulated ferritin cargo within the encapsulin nano compartment 手法: EM (単粒子) / 解像度: 5.5 Å
化合物	ChemComp-HOH: WATER
由来	Haliangium ochraceum (バクテリア) haliangium ochraceum (strain dsm 14365 / jcm 11303 / smp-2) (バクテリア)
キーワード	VIRUS LIKE PARTICLE / Encapsulin / encapsulated ferritin / haliangium ochraceum