EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for late maturation steps of the human mitoribosomal large subunit.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 3673, Year 2021
掲載日	2021年6月16日
著者	Miriam Cipullo / Genís Valentín Gesé / Anas Khawaja / B Martin Hällberg / Joanna Rorbach /
PubMed 要旨	Mitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. ...Mitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. Mitoribosome biogenesis follows distinct molecular pathways that remain poorly understood. Here, we determine the cryo-EM structures of mitoribosomes isolated from human cell lines with either depleted or overexpressed mitoribosome assembly factor GTPBP5, allowing us to capture consecutive steps during mitoribosomal large subunit (mt-LSU) biogenesis. Our structures provide essential insights into the last steps of 16S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, which require the coordinated action of nine assembly factors. We show that mammalian-specific MTERF4 contributes to the folding of 16S rRNA, allowing 16 S rRNA methylation by MRM2, while GTPBP5 and NSUN4 promote fine-tuning rRNA rearrangements leading to PTC formation. Moreover, our data reveal an unexpected involvement of the elongation factor mtEF-Tu in mt-LSU assembly, where mtEF-Tu interacts with GTPBP5, similar to its interaction with tRNA during translational elongation.
リンク	Nat Commun / PubMed:34135318 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.2 Å
構造データ	12763 7o9k EMDB-12763, PDB-7o9k: Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-Tu 手法: EM (単粒子) / 解像度: 3.1 Å 12764 7o9m EMDB-12764, PDB-7o9m: Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1 and the MALSU module 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-12767: Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-TU (MTG1-loaded subset) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-12768: Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-TU (mtEF-Tu-loaded subset) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-12769: Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1 and the MALSU module (MTG1-loaded subset) 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-12770: Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2 and the MALSU module 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SAM: S-ADENOSYLMETHIONINE ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	RIBOSOME / Mitochondria / GTPase / Ribosome assembly intermediate