EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of translation termination, rescue, and recycling in mammalian mitochondria.
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 12, Page 2566-22582.e6, Year 2021
掲載日	2021年6月17日
著者	Eva Kummer / Katharina Noel Schubert / Tanja Schoenhut / Alain Scaiola / Nenad Ban /
PubMed 要旨	The mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as ...The mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as a screening tool to identify mitochondrial translation termination mechanisms and to describe them in molecular detail. We show how mitochondrial release factor 1a releases the nascent chain from the ribosome when it encounters the canonical stop codons UAA and UAG. Furthermore, we define how the peptidyl-tRNA hydrolase ICT1 acts as a rescue factor on mitoribosomes that have stalled on truncated messages to recover them for protein synthesis. Finally, we present structural models detailing the process of mitochondrial ribosome recycling to explain how a dedicated elongation factor, mitochondrial EFG2 (mtEFG2), has specialized for cooperation with the mitochondrial ribosome recycling factor to dissociate the mitoribosomal subunits at the end of the translation process.
リンク	Mol Cell / PubMed:33878294
手法	EM (単粒子)
解像度	3.2 - 4.6 Å
構造データ	12527 7nqh EMDB-12527, PDB-7nqh: 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet 手法: EM (単粒子) / 解像度: 3.5 Å 12529 7nql EMDB-12529, PDB-7nql: 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet 手法: EM (単粒子) / 解像度: 3.4 Å 12567 7nsh EMDB-12567, PDB-7nsh: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 手法: EM (単粒子) / 解像度: 3.2 Å 12568 7nsi EMDB-12568, PDB-7nsi: 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) 手法: EM (単粒子) / 解像度: 4.6 Å 12569 7nsj EMDB-12569, PDB-7nsj: 55S mammalian mitochondrial ribosome with *tRNA(P/P) and tRNA(E) 手法: EM (単粒子) / 解像度**: 3.9 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-SPM: SPERMINE / スペルミン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-5GP: GUANOSINE-5'-MONOPHOSPHATE / GMP ChemComp-HOH: WATER ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体YM
由来	sus scrofa (ブタ) homo sapiens (ヒト) unidentified (未定義)
キーワード	TRANSLATION / Ribosome / Mitochondria / Termination / mtRF1a / Release / ICT1 / mtRRF / Recycling / tRNA(E*)