EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of early translocation events on the ribosome.
ジャーナル・号・ページ	Nature, Vol. 595, Issue 7869, Page 741-745, Year 2021
掲載日	2021年7月7日
著者	Emily J Rundlet / Mikael Holm / Magdalena Schacherl / S Kundhavai Natchiar / Roger B Altman / Christian M T Spahn / Alexander G Myasnikov / Scott C Blanchard /
PubMed 要旨	Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA selection and translocation reactions that proceed rapidly (2-20 per second) and with a low error rate (around ...Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA selection and translocation reactions that proceed rapidly (2-20 per second) and with a low error rate (around 10 to 10 at each step) over thousands of cycles. The cadence and fidelity of ribosome transit through mRNA templates in discrete codon increments is a paradigm for movement in biological systems that must hold for diverse mRNA and tRNA substrates across domains of life. Here we use single-molecule fluorescence methods to guide the capture of structures of early translocation events on the bacterial ribosome. Our findings reveal that the bacterial GTPase elongation factor G specifically engages spontaneously achieved ribosome conformations while in an active, GTP-bound conformation to unlock and initiate peptidyl-tRNA translocation. These findings suggest that processes intrinsic to the pre-translocation ribosome complex can regulate the rate of protein synthesis, and that energy expenditure is used later in the translocation mechanism than previously proposed.
リンク	Nature / PubMed:34234344 / PubMed Central
手法	EM (単粒子)
解像度	2.33 - 2.72 Å
構造データ	24120 7n1p EMDB-24120, PDB-7n1p: Elongating 70S ribosome complex in a classical pre-translocation (PRE-C) conformation 手法: EM (単粒子) / 解像度: 2.33 Å 24132 7n2c EMDB-24132, PDB-7n2c: Elongating 70S ribosome complex in a fusidic acid-stalled intermediate state of translocation bound to EF-G(GDP) (INT2) 手法: EM (単粒子) / 解像度: 2.72 Å 24133 7n2u EMDB-24133, PDB-7n2u: Elongating 70S ribosome complex in a hybrid-H1 pre-translocation (PRE-H1) conformation 手法: EM (単粒子) / 解像度: 2.53 Å 24134 7n2v EMDB-24134, PDB-7n2v: Elongating 70S ribosome complex in a spectinomycin-stalled intermediate state of translocation bound to EF-G in an active, GTP conformation (INT1) 手法: EM (単粒子) / 解像度: 2.54 Å 24135 7n30 EMDB-24135, PDB-7n30: Elongating 70S ribosome complex in a *hybrid-H2 pre-translocation (PRE-H2)* conformation 手法: EM (単粒子) / 解像度: 2.66 Å 24136 7n31 EMDB-24136, PDB-7n31: Elongating 70S ribosome complex in a post-translocation (POST) conformation 手法: EM (単粒子) / 解像度: 2.69 Å
化合物	ChemComp-PUT: 1,4-DIAMINOBUTANE / プトレシン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-HOH: WATER ChemComp-FUA: FUSIDIC ACID / フシジン酸 / 抗生剤, AntimicrobialYM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-SCM: SPECTINOMYCIN / スペクチノマイシン / 抗生剤YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM
由来	escherichia coli k-12 (大腸菌) escherichia coli bl21 (大腸菌) escherichia coli (strain k12) (大腸菌) escherichia coli (大腸菌)
キーワード	RIBOSOME / Elongation complex / tRNA / mRNA / Pre-translocation / translocation / EF-G & GTP / 70S ribosome / post-translocation