EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the Arabidopsis thaliana glutamate receptor-like channel GLR3.4.
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 15, Page 3216-33226.e8, Year 2021
掲載日	2021年8月5日
著者	Marriah N Green / Shanti Pal Gangwar / Erwan Michard / Alexander A Simon / Maria Teresa Portes / Juan Barbosa-Caro / Michael M Wudick / Michael A Lizzio / Oleg Klykov / Maria V Yelshanskaya / José A Feijó / Alexander I Sobolevsky /
PubMed 要旨	Glutamate receptor-like channels (GLRs) play vital roles in various physiological processes in plants, such as wound response, stomatal aperture control, seed germination, root development, innate ...Glutamate receptor-like channels (GLRs) play vital roles in various physiological processes in plants, such as wound response, stomatal aperture control, seed germination, root development, innate immune response, pollen tube growth, and morphogenesis. Despite the importance of GLRs, knowledge about their molecular organization is limited. Here we use X-ray crystallography and single-particle cryo-EM to solve structures of the Arabidopsis thaliana GLR3.4. Our structures reveal the tetrameric assembly of GLR3.4 subunits into a three-layer domain architecture, reminiscent of animal ionotropic glutamate receptors (iGluRs). However, the non-swapped arrangement between layers of GLR3.4 domains, binding of glutathione through S-glutathionylation of cysteine C205 inside the amino-terminal domain clamshell, unique symmetry, inter-domain interfaces, and ligand specificity distinguish GLR3.4 from representatives of the iGluR family and suggest distinct features of the GLR gating mechanism. Our work elaborates on the principles of GLR architecture and symmetry and provides a molecular template for deciphering GLR-dependent signaling mechanisms in plants.
リンク	Mol Cell / PubMed:34161757 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.5 - 4.39 Å
構造データ	23606 7lzh EMDB-23606, PDB-7lzh: Structure of the glutamate receptor-like channel AtGLR3.4 手法: EM (単粒子) / 解像度: 3.57 Å 23607 7lzi EMDB-23607, PDB-7lzi: Structure of the glutamate receptor-like channel AtGLR3.4 手法: EM (単粒子) / 解像度: 4.39 Å PDB-7lz0: Structure of glutamate receptor-like channel GLR3.4 ligand-binding domain in complex with glutamate 手法: X-RAY DIFFRACTION / 解像度: 2.29 Å PDB-7lz1: Structure of glutamate receptor-like channel GLR3.4 ligand-binding domain in complex with serine 手法: X-RAY DIFFRACTION / 解像度: 1.51 Å PDB-7lz2: Structure of glutamate receptor-like channel GLR3.4 ligand-binding domain in complex with methionine 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å
化合物	ChemComp-GLU: GLUTAMIC ACID / グルタミン酸 / グルタミン酸 ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-HOH: WATER / 水 ChemComp-SER: SERINE / セリン / セリン ChemComp-MET: METHIONINE / メチオニン / メチオニン ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-BME: BETA-MERCAPTOETHANOL / 2-メルカプトエタノ-ル / 2-メルカプトエタノール ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-GSH: GLUTATHIONE / グルタチオン / グルタチオン
由来	arabidopsis thaliana (シロイヌナズナ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Arabidopsis thaliana (シロイヌナズナ) / Ion-Channel (イオンチャネル) / glutamate receptor-like channel (GLR) / Ligand binding domain (リガンド) / TRANSPORT PROTEIN (運搬体タンパク質)