EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity.
ジャーナル・号・ページ	Science, Vol. 373, Issue 6555, Year 2021
掲載日	2021年8月6日
著者	Sophie M-C Gobeil / Katarzyna Janowska / Shana McDowell / Katayoun Mansouri / Robert Parks / Victoria Stalls / Megan F Kopp / Kartik Manne / Dapeng Li / Kevin Wiehe / Kevin O Saunders / Robert J Edwards / Bette Korber / Barton F Haynes / Rory Henderson / Priyamvada Acharya /
PubMed 要旨	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with multiple spike mutations enable increased transmission and antibody resistance. We combined cryo-electron microscopy (cryo- ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with multiple spike mutations enable increased transmission and antibody resistance. We combined cryo-electron microscopy (cryo-EM), binding, and computational analyses to study variant spikes, including one that was involved in transmission between minks and humans, and others that originated and spread in human populations. All variants showed increased angiotensin-converting enzyme 2 (ACE2) receptor binding and increased propensity for receptor binding domain (RBD)-up states. While adaptation to mink resulted in spike destabilization, the B.1.1.7 (UK) spike balanced stabilizing and destabilizing mutations. A local destabilizing effect of the RBD E484K mutation was implicated in resistance of the B.1.1.28/P.1 (Brazil) and B.1.351 (South Africa) variants to neutralizing antibodies. Our studies revealed allosteric effects of mutations and mechanistic differences that drive either interspecies transmission or escape from antibody neutralization.
リンク	Science / PubMed:34168071 / PubMed Central
手法	EM (単粒子)
解像度	2.83 - 4.05 Å
構造データ	23546 7lwi EMDB-23546, PDB-7lwi: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 3-RBD down conformation 手法: EM (単粒子) / 解像度: 3.07 Å 23547 7lwj EMDB-23547, PDB-7lwj: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 3-RBD down conformation 手法: EM (単粒子) / 解像度: 3.24 Å 23548 7lwk EMDB-23548, PDB-7lwk: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 3-RBD down conformation 手法: EM (単粒子) / 解像度: 2.92 Å 23549 7lwl EMDB-23549, PDB-7lwl: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 3-RBD down conformation 手法: EM (単粒子) / 解像度: 2.84 Å 23550 7lwm EMDB-23550, PDB-7lwm: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 1-RBD up conformation 手法: EM (単粒子) / 解像度: 2.83 Å 23551 7lwn EMDB-23551, PDB-7lwn: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 1-RBD up conformation 手法: EM (単粒子) / 解像度: 2.94 Å 23552 7lwo EMDB-23552, PDB-7lwo: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 1-RBD up conformation 手法: EM (単粒子) / 解像度: 2.85 Å 23553 7lwp EMDB-23553, PDB-7lwp: Mink Cluster 5-associated SARS-CoV-2 spike protein (S-GSAS-D614G-delFV) in the 2-RBD up conformation 手法: EM (単粒子) / 解像度: 3.01 Å 23554 7lwq EMDB-23554, PDB-7lwq: Mink Cluster 5-associated SARS-CoV-2 spike protein(S-GSAS-D614G-delFV) missing the S1 subunit and SD2 subdomain of one protomer 手法: EM (単粒子) / 解像度: 3.44 Å 23556 7lwt EMDB-23556, PDB-7lwt: UK (B.1.1.7) SARS-CoV-2 spike protein variant (S-GSAS-B.1.1.7) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.19 Å 23557 7lwu EMDB-23557, PDB-7lwu: UK (B.1.1.7) SARS-CoV-2 spike protein variant (S-GSAS-B.1.1.7) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.22 Å 23558 7lwv EMDB-23558, PDB-7lwv: UK (B.1.1.7) SARS-CoV-2 spike protein variant (S-GSAS-B.1.1.7) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.12 Å 23559 7lww EMDB-23559, PDB-7lww: Triple mutant (K417N-E484K-N501Y) SARS-CoV-2 spike protein in the 1-RBD-up conformation (S-GSAS-D614G-K417N-E484K-N501Y) 手法: EM (単粒子) / 解像度: 3.0 Å 23593 7lyk EMDB-23593, PDB-7lyk: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the 2-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.65 Å 23594 7lyl EMDB-23594, PDB-7lyl: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the RBD-down conformation 手法: EM (単粒子) / 解像度: 3.72 Å 23595 7lym EMDB-23595, PDB-7lym: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the RBD-down conformation 手法: EM (単粒子) / 解像度: 3.57 Å 23596 7lyn EMDB-23596, PDB-7lyn: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.32 Å 23597 7lyo EMDB-23597, PDB-7lyo: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.32 Å 23598 7lyp EMDB-23598, PDB-7lyp: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 4.05 Å 23599 7lyq EMDB-23599, PDB-7lyq: South African (B.1.351) SARS-CoV-2 spike protein variant (S-GSAS-B.1.351) in the 1-RBD-up conformation 手法: EM (単粒子) / 解像度: 3.34 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス)
キーワード	VIRAL PROTEIN / SARS-CoV-2 Spike Protein Trimer