+検索条件
-Structure paper
タイトル | Prefusion structure of human cytomegalovirus glycoprotein B and structural basis for membrane fusion. |
---|---|
ジャーナル・号・ページ | Sci Adv, Vol. 7, Issue 10, Year 2021 |
掲載日 | 2021年3月5日 |
著者 | Yuhang Liu / Kyle P Heim / Ye Che / Xiaoyuan Chi / Xiayang Qiu / Seungil Han / Philip R Dormitzer / Xinzhen Yang / |
PubMed 要旨 | Human cytomegalovirus (HCMV) causes congenital disease with long-term morbidity. HCMV glycoprotein B (gB) transitions irreversibly from a metastable prefusion to a stable postfusion conformation to ...Human cytomegalovirus (HCMV) causes congenital disease with long-term morbidity. HCMV glycoprotein B (gB) transitions irreversibly from a metastable prefusion to a stable postfusion conformation to fuse the viral envelope with a host cell membrane during entry. We stabilized prefusion gB on the virion with a fusion inhibitor and a chemical cross-linker, extracted and purified it, and then determined its structure to 3.6-Å resolution by electron cryomicroscopy. Our results revealed the structural rearrangements that mediate membrane fusion and details of the interactions among the fusion loops, the membrane-proximal region, transmembrane domain, and bound fusion inhibitor that stabilized gB in the prefusion state. The structure rationalizes known gB antigenic sites. By analogy to successful vaccine antigen engineering approaches for other viral pathogens, the high-resolution prefusion gB structure provides a basis to develop stabilized prefusion gB HCMV vaccine antigens. |
リンク | Sci Adv / PubMed:33674318 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.5 - 3.6 Å |
構造データ | EMDB-22819, PDB-7kdd: EMDB-22828, PDB-7kdp: |
化合物 | ChemComp-NAG: ChemComp-CA: ChemComp-WCY: |
由来 |
|
キーワード | VIRAL PROTEIN/IMMUNE SYSTEM / fusogen / postfusion / HCMV / gB / antibody / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex / VIRAL PROTEIN/INHIBITOR / prefusion / fusion inhibitor / VIRAL PROTEIN-INHIBITOR complex |