EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Stepwise gating of the Sec61 protein-conducting channel by Sec63 and Sec62.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 2, Page 162-172, Year 2021
掲載日	2021年1月4日
著者	Samuel Itskanov / Katie M Kuo / James C Gumbart / Eunyong Park /
PubMed 要旨	Many proteins are transported into the endoplasmic reticulum by the universally conserved Sec61 channel. Post-translational transport requires two additional proteins, Sec62 and Sec63, but their ...Many proteins are transported into the endoplasmic reticulum by the universally conserved Sec61 channel. Post-translational transport requires two additional proteins, Sec62 and Sec63, but their functions are poorly defined. In the present study, we determined cryo-electron microscopy (cryo-EM) structures of several variants of Sec61-Sec62-Sec63 complexes from Saccharomyces cerevisiae and Thermomyces lanuginosus and show that Sec62 and Sec63 induce opening of the Sec61 channel. Without Sec62, the translocation pore of Sec61 remains closed by the plug domain, rendering the channel inactive. We further show that the lateral gate of Sec61 must first be partially opened by interactions between Sec61 and Sec63 in cytosolic and luminal domains, a simultaneous disruption of which completely closes the channel. The structures and molecular dynamics simulations suggest that Sec62 may also prevent lipids from invading the channel through the open lateral gate. Our study shows how Sec63 and Sec62 work together in a hierarchical manner to activate Sec61 for post-translational protein translocation.
リンク	Nat Struct Mol Biol / PubMed:33398175 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 4.4 Å
構造データ	22770 7kah EMDB-22770, PDB-7kah: Cryo-EM structure of the Sec complex from S. cerevisiae, wild-type, class without Sec62 手法: EM (単粒子) / 解像度: 3.1 Å 22771 7kai EMDB-22771, PDB-7kai: Cryo-EM structure of the Sec complex from S. cerevisiae, wild-type, class with Sec62, conformation 1 (C1) 手法: EM (単粒子) / 解像度: 3.2 Å 22772 7kaj EMDB-22772, PDB-7kaj: Cryo-EM structure of the Sec complex from S. cerevisiae, wild-type, class with Sec62, conformation 2 (C2) 手法: EM (単粒子) / 解像度: 3.1 Å 22773 7kak EMDB-22773, PDB-7kak: Cryo-EM structure of the Sec complex from T. lanuginosus, wild-type, class without Sec62 手法: EM (単粒子) / 解像度: 3.9 Å 22774 7kal EMDB-22774, PDB-7kal: Cryo-EM structure of the Sec complex from T. lanuginosus, wild-type, class with Sec62, plug-open conformation 手法: EM (単粒子) / 解像度: 4.0 Å 22775 7kam EMDB-22775, PDB-7kam: Cryo-EM structure of the Sec complex from T. lanuginosus, wild-type, class with Sec62, plug-closed conformation 手法: EM (単粒子) / 解像度: 3.8 Å 22776 7kan EMDB-22776: Cryo-EM structure of the Sec complex from T. lanuginosus lacking Sec62 PDB-7kan: Cryo-EM structure of the Sec complex from T. lanuginosus, Sec62-lacking mutant (Delta Sec62) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-22777: Cryo-EM structure of the Sec complex from T. lanuginosus, Sec62 anchor domain mutant (delta anchor) 手法: EM (単粒子) / 解像度: 4.4 Å 22778 7kao EMDB-22778, PDB-7kao: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore mutant, class without Sec62 手法: EM (単粒子) / 解像度: 4.0 Å 22779 7kap EMDB-22779, PDB-7kap: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore mutant, class with Sec62, conformation 1 (C1) 手法: EM (単粒子) / 解像度: 4.1 Å 22780 7kaq EMDB-22780, PDB-7kaq: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore mutant, class with Sec62, conformation 2 (C2) 手法: EM (単粒子) / 解像度: 4.0 Å 22781 7kar EMDB-22781, PDB-7kar: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec63 FN3 mutant, class without Sec62 手法: EM (単粒子) / 解像度: 4.0 Å 22782 7kas EMDB-22782, PDB-7kas: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec63 FN3 mutant, class with Sec62 手法: EM (単粒子) / 解像度: 3.9 Å 22783 7kat EMDB-22783, PDB-7kat: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore ring and Sec63 FN3 double mutant, class without Sec62 手法: EM (単粒子) / 解像度: 4.4 Å 22784 7kau EMDB-22784, PDB-7kau: Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore ring and Sec63 FN3 double mutant, class with Sec62 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-22785: Cryo-EM structure of the Sec complex from S. cerevisiae, wild-type, consensus map 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-22786: Cryo-EM structure of the Sec complex from T. lanuginosus, wild-type, consensus map of classes with Sec62 手法: EM (単粒子) / 解像度: 3.75 Å 22787 7kb5 EMDB-22787, PDB-7kb5: Cryo-EM structure of the Sec complex from yeast, Sec63 FN3 and residues 210-216 mutated 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質*YM
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) saccharomyces cerevisiae by4741 (パン酵母) thermomyces lanuginosus (菌類)
キーワード	PROTEIN TRANSPORT / Sec61 / translocon / endoplasmic reticulum / protein translocation / Sec62 / Sec63 / channel