EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of the mycobacterial membrane protein MmpL3 reveal its mechanism of lipid transport.
ジャーナル・号・ページ	PLoS Biol, Vol. 19, Issue 8, Page e3001370, Year 2021
掲載日	2021年8月12日
著者	Chih-Chia Su / Philip A Klenotic / Meng Cui / Meinan Lyu / Christopher E Morgan / Edward W Yu /
PubMed 要旨	The mycobacterial membrane protein large 3 (MmpL3) transporter is essential and required for shuttling the lipid trehalose monomycolate (TMM), a precursor of mycolic acid (MA)-containing trehalose ...The mycobacterial membrane protein large 3 (MmpL3) transporter is essential and required for shuttling the lipid trehalose monomycolate (TMM), a precursor of mycolic acid (MA)-containing trehalose dimycolate (TDM) and mycolyl arabinogalactan peptidoglycan (mAGP), in Mycobacterium species, including Mycobacterium tuberculosis and Mycobacterium smegmatis. However, the mechanism that MmpL3 uses to facilitate the transport of fatty acids and lipidic elements to the mycobacterial cell wall remains elusive. Here, we report 7 structures of the M. smegmatis MmpL3 transporter in its unbound state and in complex with trehalose 6-decanoate (T6D) or TMM using single-particle cryo-electron microscopy (cryo-EM) and X-ray crystallography. Combined with calculated results from molecular dynamics (MD) and target MD simulations, we reveal a lipid transport mechanism that involves a coupled movement of the periplasmic domain and transmembrane helices of the MmpL3 transporter that facilitates the shuttling of lipids to the mycobacterial cell wall.
リンク	PLoS Biol / PubMed:34383749 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.66 - 4.33 Å
構造データ	22724 7k8a EMDB-22724, PDB-7k8a: CryoEM structure of a trehalose monomycolate transporter in lipid nanodiscs 手法: EM (単粒子) / 解像度: 3.65 Å 22725 7k8b EMDB-22725, PDB-7k8b: CryoEM structure of a trehalose monomycolate transporter in lipid nanodiscs 手法: EM (単粒子) / 解像度: 2.94 Å 22726 7k8c EMDB-22726, PDB-7k8c: CryoEM structure of a trehalose monomycolate transporter in lipid nanodiscs 手法: EM (単粒子) / 解像度: 4.27 Å 22728 7k8d EMDB-22728, PDB-7k8d: CryoEM structure of a trehalose monomycolate transporter in TMM lipid nanodiscs (form II) 手法: EM (単粒子) / 解像度: 4.33 Å 24206 7n6b EMDB-24206, PDB-7n6b: Structure of MmpL3 reconstituted into lipid nanodisc in the TMM bound state 手法: EM (単粒子) / 解像度: 2.66 Å PDB-7k7m: Crystal Structure of a membrane protein 手法: X-RAY DIFFRACTION / 解像度: 3.33 Å
化合物	ChemComp-0HJ: 6-O-[(2S)-2-{(1S)-18-[(1R,2R)-2-hexylcyclopropyl]-1-hydroxyoctadecyl}tricosanoyl]-alpha-D-glucopyranosyl alpha-D-glucopyranoside
由来	mycolicibacterium smegmatis (バクテリア) mycolicibacterium smegmatis (strain atcc 700084 / mc(2)155) (バクテリア)
キーワード	MEMBRANE PROTEIN / transporter / TRANSLOCASE / trehalose monomycolate transporter