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-Structure paper
タイトル | Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 49, Issue 1, Page 547-567, Year 2021 |
掲載日 | 2021年1月11日 |
著者 | Vikash Jha / Bappaditya Roy / Dushyant Jahagirdar / Zakkary A McNutt / Elan A Shatoff / Bethany L Boleratz / Dean E Watkins / Ralf Bundschuh / Kaustuv Basu / Joaquin Ortega / Kurt Fredrick / |
PubMed 要旨 | Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the ...Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the Bacteroidetes, fail to recognize the SD sequence of mRNA in vitro. A cryo-electron microscopy structure of the complete 70S ribosome from F. johnsoniae at 2.8 Å resolution reveals that the ASD is sequestered by ribosomal proteins bS21, bS18 and bS6, explaining the basis of ASD inhibition. The structure also uncovers a novel ribosomal protein-bL38. Remarkably, in F. johnsoniae and many other Flavobacteriia, the gene encoding bS21 contains a strong SD, unlike virtually all other genes. A subset of Flavobacteriia have an alternative ASD, and in these organisms the fully complementary sequence lies upstream of the bS21 gene, indicative of natural covariation. In other Bacteroidetes classes, strong SDs are frequently found upstream of the genes for bS21 and/or bS18. We propose that these SDs are used as regulatory elements, enabling bS21 and bS18 to translationally control their own production. |
リンク | Nucleic Acids Res / PubMed:33330920 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 Å |
構造データ | EMDB-22345, PDB-7jil: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME (リボソーム) / Translation Initiation / Protein synthesis (タンパク質生合成) / bS21 |